| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human FABP12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于FABP12抗体的示例参考文献(注：文献信息为虚构示例，实际需查询学术数据库获取真实文献)：
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1. **"Characterization of a Novel Polyclonal Antibody Against FABP12 for Lipid Metabolism Studies"**
*作者：Smith et al. (2020)*
**摘要**：开发了针对FABP12的多克隆抗体，通过Western blot和免疫组化验证其特异性，发现FABP12在小鼠视网膜和脂肪组织中高表达，提示其可能在脂质转运中发挥作用。
2. **"FABP12 Expression in Adipose Tissue: Insights from Immunohistochemical Analysis"**
*作者：Johnson & Lee (2018)*
**摘要**：利用新制备的FABP12单克隆抗体，揭示了其在哺乳动物脂肪细胞中的亚细胞定位，并发现其表达与肥胖模型中的代谢紊乱相关。
3. **"Role of FABP12 in Retinal Function: Evidence from Antibody-Based Knockdown Models"**
*作者：Chen et al. (2021)*
**摘要**：通过抗FABP12抗体抑制蛋白功能，发现其缺失导致视网膜光感受器细胞脂质代谢异常，暗示FABP12对视觉健康的关键作用。
4. **"Development of a High-Specificity FABP12 Antibody for Diagnostic Applications"**
*作者：Wang et al. (2019)*
**摘要**：报道了一种高灵敏度FABP12抗体，通过ELISA和流式细胞术验证，可用于血清中FABP12的定量检测，潜在应用于代谢综合征的生物标志物研究。
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建议通过 **PubMed** 或 **Web of Science** 检索真实文献，关键词如“FABP12 antibody”或“FABP12 characterization”。
Fatty acid-binding protein 12 (FABP12) is a member of the FABP family, which comprises intracellular lipid chaperones involved in fatty acid uptake, transport, and metabolic regulation. While extensively studied members like FABP4 (adipocyte) or FABP1 (liver) are well-characterized, FABP12 remains less understood. It shares the conserved β-barrel structure typical of FABPs, enabling hydrophobic ligand binding, but exhibits distinct tissue expression patterns. Emerging evidence suggests FABP12 may localize to tissues such as testis, adipose, or intestinal regions, though its precise distribution and physiological roles require further validation.
FABP12 antibodies are critical tools for investigating its expression, subcellular localization, and interaction networks. Polyclonal antibodies often target unique peptide sequences within its variable regions to ensure specificity, while monoclonal antibodies enable reproducible detection in assays like Western blot, immunohistochemistry, or immunofluorescence. These reagents help elucidate FABP12's potential roles in lipid metabolism, cellular signaling, or disease contexts. For instance, dysregulation of FABPs is linked to metabolic disorders, inflammation, and cancer, making FABP12 a candidate biomarker or therapeutic target.
However, challenges persist due to FABP12's structural homology with other family members, necessitating rigorous antibody validation via knockout controls or epitope mapping. Current research focuses on clarifying its ligand preferences, tissue-specific functions, and relevance in pathologies. Reliable FABP12 antibodies thus remain pivotal for advancing its biological and clinical characterization.
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