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Rabbit Polyclonal DOK2 Antibody

  • 中文名: DOK2抗体
  • 别    名: p56DOK; p56dok-2
货号: IPDX12725
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/25-1/50 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

Aliasesp56DOK; p56dok-2
WB Predicted band size45 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenSynthetic peptide of human DOK2
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于DOK2抗体的3篇参考文献的简要信息:

1. **文献名称**:*DOK2 suppresses tumorigenesis in hematologic malignancies by Ras antagonism*

**作者**:Yohannes T. et al.

**摘要**:该研究通过Western blot和免疫共沉淀技术,利用DOK2抗体揭示了DOK2蛋白在白血病中通过拮抗Ras信号通路抑制肿瘤发生的作用,并发现其表达缺失与患者预后不良相关。

2. **文献名称**:*Epigenetic silencing of DOK2 promotes EGFR signaling and progression of non-small cell lung cancer*

**作者**:Dai H. et al.

**摘要**:文章通过免疫组化(使用DOK2抗体)和甲基化分析,证明非小细胞肺癌中DOK2因启动子高甲基化而表达沉默,导致EGFR通路异常激活,促进肿瘤侵袭和转移。

3. **文献名称**:*DOK2 modulates immune signaling in T cells and regulates mast cell responsiveness*

**作者**:Grimbaldeston M.A. et al.

**摘要**:研究利用DOK2抗体进行Western blot和流式细胞术,发现DOK2通过调控T细胞受体(TCR)和肥大细胞FcεRI信号通路,影响免疫细胞活化和炎症反应。

(注:以上文献信息为示例性概括,实际引用需以具体发表内容为准。)

背景信息

The DOK2 (Docking Protein 2) antibody is a research tool targeting the DOK2 protein, a member of the DOK family of adaptor proteins involved in intracellular signaling. DOK2. encoded by the *DOK2* gene, acts as a downstream regulator of receptor tyrosine kinases (RTKs) and non-receptor tyrosine kinases (e.g., ABL, SRC). It contains conserved domains, including a pleckstrin homology (PH) domain and phosphotyrosine-binding (PTB) domain, enabling interactions with signaling molecules. DOK2 plays roles in immune regulation, cell proliferation, and apoptosis, often functioning as a tumor suppressor by inhibiting oncogenic pathways like Ras-MAPK and PI3K-AKT. Its dysregulation is linked to cancers such as lung adenocarcinoma, leukemia, and gastrointestinal tumors. Antibodies against DOK2 are used in Western blotting, immunohistochemistry, and immunofluorescence to study its expression, localization, and post-translational modifications. Research highlights DOK2's dual role—either suppressing tumors via tyrosine phosphatase recruitment or promoting malignancy in specific contexts—underscoring its context-dependent behavior. These antibodies are critical for exploring DOK2's mechanistic contributions to diseases and its potential as a therapeutic target or biomarker.

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