| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HM2 |
| WB Predicted band size | 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human CHRM2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CHRM2抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*"Selective activation of M2 muscarinic receptors in myocardial ischemia: Role of inhibitory G proteins and extracellular signal-regulated kinases"*
**作者**：Wang H, Shi H, Zhang L.
**摘要**：该研究使用CHRM2特异性抗体，通过免疫印迹和免疫组织化学技术，揭示了心肌缺血模型中M2受体与抑制性G蛋白(Gi/o)及ERK信号通路的关联，表明CHRM2激活对心脏保护具有潜在作用。
2. **文献名称**：*"Cholinergic receptor M2 (CHRM2) in Alzheimer’s disease: Immunohistochemical localization and synaptic integrity analysis"*
**作者**：Elsayed NM, Decker MJ, Morrison-Bogorad M.
**摘要**：利用CHRM2抗体对阿尔茨海默病患者脑组织进行免疫染色，发现海马区CHRM2表达显著降低，且与突触标志物Synaptophysin的丢失相关，提示其与认知功能障碍的联系。
3. **文献名称**：*"Antibody-based profiling of M2 muscarinic receptor expression in peripheral blood mononuclear cells: Implications for psychiatric disorders"*
**作者**：Jones RM, O’Brien WT, Scarr E, Dean B.
**摘要**：通过流式细胞术和ELISA结合CHRM2抗体，研究发现精神分裂症患者外周血单核细胞中CHRM2蛋白水平异常升高，可能反映外周与中枢胆碱能系统失调的关联。
(注：上述文献为示例性内容，实际引用时需核实真实论文信息。)
The CHRM2 antibody targets the cholinergic muscarinic acetylcholine receptor M2 (CHRM2), a G protein-coupled receptor (GPCR) critical in mediating parasympathetic nervous system responses. CHRM2 is predominantly expressed in the heart, smooth muscles, and specific regions of the central nervous system. It regulates heart rate by slowing sinoatrial node activity, influences smooth muscle contraction, and modulates cognitive functions such as memory and learning. Dysregulation of CHRM2 has been linked to cardiovascular disorders, neurodegenerative diseases, and psychiatric conditions, making it a key focus in pharmacological research.
CHRM2 antibodies are essential tools for studying receptor localization, expression levels, and functional interactions. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to visualize receptor distribution in tissues or cultured cells. Specificity and validation of these antibodies are crucial, often confirmed using CHRM2 knockout models or competitive binding assays. Researchers also employ CHRM2 antibodies to explore receptor signaling pathways, such as Gi/o protein coupling and downstream effectors like adenylate cyclase.
Recent studies highlight CHRM2's role in diseases like atrial fibrillation, Alzheimer's disease, and schizophrenia, driving interest in therapeutic targeting. However, challenges remain in developing selective ligands due to structural similarities among muscarinic receptor subtypes. CHRM2 antibodies thus serve as vital reagents for both basic research and drug discovery efforts.
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