| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | AK3; AK 4; AK3L1; AK3L2 |
| WB Predicted band size | 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human AK4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于AK4抗体的3篇参考文献摘要概览：
1. **标题**：*Adenylate kinase 4 modulates oxidative phosphorylation and promotes colorectal cancer progression*
**作者**：Zhang Y, et al.
**摘要**：该研究通过免疫组化和Western blot发现AK4在结直肠癌中高表达，其通过调节线粒体氧化磷酸化促进肿瘤细胞增殖和转移，敲低AK4可抑制小鼠模型肿瘤生长。
2. **标题**：*AK4 deficiency enhances chemosensitivity by regulating mitochondrial function in hepatocellular carcinoma*
**作者**：Li X, et al.
**摘要**：研究利用AK4特异性抗体检测肝癌患者样本，发现AK4低表达与化疗敏感性相关。机制上，AK4缺失导致线粒体膜电位下降，促进凋亡通路激活，增强顺铂疗效。
3. **标题**：*AK4 as a prognostic biomarker in non-small cell lung cancer: Correlation with PD-L1 expression*
**作者**：Chen H, et al.
**摘要**：通过免疫荧光共定位分析，发现AK4与PD-L1在肺癌组织中共表达，高AK4水平提示患者预后不良，可能通过调控HIF-1α通路促进免疫逃逸。
注：以上为示例性内容，实际文献需通过PubMed/Google Scholar检索确认。建议补充检索关键词如“AK4 antibody application”或“AK4 cancer therapeutic target”获取具体实验数据。
Adenylate kinase 4 (AK4) is a mitochondrial enzyme belonging to the adenylate kinase family, which regulates nucleotide metabolism by catalyzing the reversible transfer of phosphate groups between nucleotides (e.g., ATP + AMP ↔ 2 ADP). AK4 is distinct from other isoforms due to its preferential localization in mitochondria and its role in maintaining energy homeostasis under stress conditions. It is ubiquitously expressed but shows elevated levels in tissues with high metabolic demands, such as the liver, heart, and brain.
AK4 has gained attention for its involvement in cellular adaptation to hypoxia, oxidative stress, and metabolic reprogramming. Studies link its dysregulation to cancer progression, neurodegenerative diseases, and metabolic disorders. In cancers, AK4 overexpression is associated with enhanced tumor cell survival, metastasis, and chemoresistance, likely through modulating mitochondrial function and reactive oxygen species (ROS) signaling.
AK4-specific antibodies are essential tools for detecting protein expression, localization, and functional studies. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore AK4's role in disease mechanisms. Recent research also investigates AK4 as a potential diagnostic biomarker or therapeutic target, particularly in cancers where metabolic alterations drive pathogenesis. However, its precise molecular interactions and regulatory pathways remain under active investigation, highlighting the need for high-specificity AK4 antibodies in advancing both basic and translational research.
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