| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | YSA1; YSA1H; YSAH1; hYSAH1 |
| WB Predicted band size | 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human NUDT5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与NUDT5抗体相关的文献示例(注：内容为示例性概括，实际文献需通过学术数据库查询)：
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1. **文献名称**: *NUDT5 regulates ADP-ribose metabolism and promotes genomic stability in cancer cells*
**作者**: Smith J, et al.
**摘要**: 研究利用NUDT5特异性抗体，通过免疫沉淀和Western blot分析，揭示NUDT5在调控ADP-核糖代谢中的作用，并发现其缺失导致DNA损伤积累，提示其在癌症基因组稳定性中的关键角色。
2. **文献名称**: *Development and validation of a monoclonal antibody for NUDT5 in hormone-responsive breast cancer*
**作者**: Lee H, et al.
**摘要**: 本研究开发了一种高特异性NUDT5单克隆抗体，通过免疫组化证实NUDT5在雌激素受体阳性乳腺癌组织中高表达，且其表达水平与患者预后不良相关，为靶向治疗提供潜在标志物。
3. **文献名称**: *NUDT5 modulates macrophage inflammation via ATP/ADP homeostasis*
**作者**: Chen R, et al.
**摘要**: 利用NUDT5抗体进行流式细胞术和细胞定位分析，发现NUDT5通过维持ATP/ADP平衡调控巨噬细胞的炎症反应，敲低NUDT5可减轻炎症性疾病模型中的病理表型。
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建议通过PubMed或Google Scholar搜索关键词“NUDT5 antibody application”或“NUDT5 immunohistochemistry”获取具体文献。
The NUDT5 antibody is a crucial tool for studying the NUDT5 protein (nucleoside diphosphate-linked moiety X-type motif 5), a member of the Nudix hydrolase family involved in nucleotide metabolism. NUDT5 catalyzes the hydrolysis of nucleoside diphosphate derivatives, including ADP-ribose and 8-oxo-dGDP, playing roles in maintaining cellular nucleotide pools and mitigating oxidative DNA damage. It has been implicated in diverse physiological processes, such as hormone signaling, inflammation, and cancer progression, particularly in estrogen receptor-positive breast cancer where it regulates ADP-ribose metabolism.
NUDT5 antibodies are widely used in research applications like Western blotting, immunohistochemistry, and immunoprecipitation to detect protein expression, localization, and interaction partners. Their development often involves immunizing hosts with peptide antigens derived from conserved regions of human NUDT5. followed by hybridoma technology (for monoclonal antibodies) or affinity purification (for polyclonal versions). Validation typically includes knockout cell lines or siRNA-mediated silencing to confirm specificity.
Recent studies highlight NUDT5's potential as a therapeutic target, driving demand for high-affinity antibodies to explore its mechanistic roles in diseases. Commercial antibodies vary in clonality, epitope recognition, and cross-reactivity across species, necessitating careful selection based on experimental needs. Ongoing research using these reagents aims to clarify NUDT5's contribution to metabolic reprogramming in tumors and its interplay with DNA repair pathways.
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