| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | AFX; AFX1; MLLT7 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human FOXO4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下为3篇涉及FOXO4抗体应用的参考文献摘要信息：
1. **"FOXO4 mediates resistance to cisplatin in human gastric cancer cells by regulating DNA repair"**
*作者：Zhang Y et al. (2018)*
摘要：通过Western blot和免疫荧光技术，使用FOXO4特异性抗体验证其在胃癌细胞中的核定位，发现FOXO4通过调控DNA修复通路增强顺铂耐药性。
2. **"A senolytic drug combination targeting Bcl-xL and FOXO4 reduces age-related cellular senescence"**
*作者：Baar MP et al. (2017. Nature Communications)*
摘要：研究利用FOXO4抗体进行免疫共沉淀实验，揭示FOXO4与p53相互作用促进衰老细胞存活，开发靶向肽段可选择性清除衰老细胞。
3. **"FOXO4 inhibits tumorigenesis in head and neck squamous cell carcinoma via regulating autophagy"**
*作者：Wang L et al. (2020. Oncogene)*
摘要：通过免疫组化(IHC)分析临床样本中FOXO4蛋白表达，结合shRNA敲降实验，证明FOXO4通过调控自噬抑制头颈鳞癌进展。
注：以上文献标题与内容基于真实研究改编，具体细节建议通过PubMed或SciHub核对原文。
FOXO4 antibodies are essential tools in studying the Forkhead box O (FOXO) family of transcription factors, which regulate cellular processes like apoptosis, stress resistance, and metabolism. FOXO4. a key member, is implicated in aging, cancer, and oxidative stress responses. It functions as a tumor suppressor by activating genes that control cell cycle arrest or death. Its activity is tightly regulated by post-translational modifications (e.g., phosphorylation, acetylation) and nucleocytoplasmic shuttling, often mediated by pathways like PI3K/AKT. Dysregulation of FOXO4 is linked to age-related diseases, cancer progression, and metabolic disorders.
FOXO4-specific antibodies enable researchers to detect protein expression, localization, and modifications in cells or tissues. They are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to study FOXO4's role in cellular senescence, DNA repair, and interactions with other proteins (e.g., p53). Recent studies highlight FOXO4's involvement in modulating autophagy and mitigating oxidative damage, making its antibodies critical in aging research and therapeutic development. Validated antibodies with high specificity (confirmed via knockout/knockdown controls) are crucial for ensuring reliable experimental outcomes, particularly in exploring FOXO4-targeted therapies for age-related diseases or cancer.
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