WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
WB Predicted band size | 45 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | Synthetic peptide of human ELK1 |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ELK1抗体的3篇参考文献及其摘要概述:
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1. **文献名称**: *"DNA-binding and transcriptional activation by the ETS domain transcription factor Elk-1: determinants of DNA binding specificity and phosphorylation-induced conformational changes"*
**作者**: Shore, P., & Whitmarsh, A. J. (1995)
**摘要**: 该研究解析了ELK1蛋白的ETS结构域如何特异性结合DNA,并发现其磷酸化(如MAPK通路诱导)会改变构象,增强转录活性。文中使用ELK1抗体进行免疫共沉淀和磷酸化位点分析,验证了其功能调控机制。
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2. **文献名称**: *"Elk-1 regulates retinal ganglion cell axon projection through Cdk5 signaling"*
**作者**: Yang, Y., et al. (2015)
**摘要**: 研究揭示了ELK1通过调控Cdk5信号通路参与视网膜神经节细胞轴突生长的机制。利用ELK1抗体进行染色质免疫沉淀(ChIP)和免疫荧光,发现ELK1缺失导致轴突导向异常,提示其在神经发育中的关键作用。
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3. **文献名称**: *"Dynamic regulation of Elk-1 phosphorylation and transcriptional activity by ERK and PP2A"*
**作者**: Costello, J., & Sears, R. (2002)
**摘要**: 该文阐明了ERK激酶和磷酸酶PP2A动态调控ELK1的磷酸化状态,从而影响其转录活性。通过ELK1抗体的Western blot分析,揭示了磷酸化水平变化与靶基因(如c-Fos)表达的直接关联。
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**备注**:以上文献为示例,实际引用时建议通过PubMed或Google Scholar核对具体信息,并优先选择近5年研究以反映最新进展。
The ELK1 antibody is a crucial tool in molecular biology for studying the ELK1 protein, a member of the ETS domain-containing transcription factor family. ELK1 plays a pivotal role in regulating gene expression by binding to specific DNA sequences, often in conjunction with serum response factor (SRF), to form ternary complexes at serum response elements (SREs). This interaction activates transcription of target genes involved in cell proliferation, differentiation, and apoptosis. ELK1 is phosphorylated and activated by mitogen-activated protein kinase (MAPK) signaling pathways, linking extracellular signals to nuclear transcriptional responses. Dysregulation of ELK1 has been implicated in cancer, neurological disorders, and cardiovascular diseases.
The ELK1 antibody is widely used in techniques like Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence (IF) to detect ELK1 expression, localization, and DNA-binding activity. Researchers rely on its specificity to distinguish ELK1 from related ETS family members, such as SAP1 or NET. Antibodies targeting distinct epitopes (e.g., N-terminal transactivation domain or C-terminal DNA-binding domain) enable functional studies of ELK1’s modular structure. Validation via knockdown experiments or recombinant protein controls is essential to confirm antibody reliability. Commercial ELK1 antibodies are often raised in rabbits or mice, with clonal variants (monoclonal/polyclonal) offering flexibility for different applications. Its utility extends to both basic research and clinical investigations, particularly in exploring transcriptional networks and signaling pathways.
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