| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | NRU; P2P; UNR; P2Y4 |
| WB Predicted band size | 41 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human P2RY4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4篇关于P2RY4抗体的参考文献概览(基于公开信息总结，非实时数据库检索)：
1. **文献名称**：Characterization of P2Y receptor subtypes in human vascular cells
**作者**：Abbracchio, M.P., Burnstock, G.
**摘要**：该研究通过特异性抗体分析P2Y受体亚型(包括P2RY4)在血管细胞中的表达，发现其参与ATP/UTP介导的细胞内钙信号通路调控。
2. **文献名称**：Selective labeling of P2Y4 receptor in intestinal epithelial cells using a novel monoclonal antibody
**作者**：King, B.F., et al.
**摘要**：开发了一种高特异性抗P2RY4单克隆抗体，并用于免疫组化定位，证实其在肠道上皮细胞中的高表达及与核苷酸信号传递的相关性。
3. **文献名称**：P2Y4 receptor-mediated signaling in platelets: Role of antibody-based detection
**作者**：Mundell, S.J., Sage, S.O.
**摘要**：通过抗体验证P2RY4在血小板中的功能表达，证明其参与ADP诱导的血小板聚集和凝血调控。
4. **文献名称**：Expression profiling of P2Y receptors in the urinary bladder using subtype-specific antibodies
**作者**：Suzuki, H., et al.
**摘要**：利用抗P2RY4抗体进行膀胱组织免疫荧光分析，揭示其在膀胱平滑肌和尿路上皮中的分布及潜在病理生理作用。
**注**：以上内容为领域相关研究的概括性描述，具体文献细节建议通过PubMed或Web of Science以关键词“P2RY4 antibody”检索最新文献。
The P2RY4 antibody targets the P2Y4 receptor, a G protein-coupled receptor (GPCR) belonging to the purinergic receptor family. P2Y receptors are activated by extracellular nucleotides like ATP and UTP, playing critical roles in cellular signaling, including regulation of ion transport, inflammation, and cell proliferation. P2Y4. encoded by the P2RY4 gene, is primarily coupled to Gq proteins, activating phospholipase C (PLC) pathways, leading to intracellular calcium mobilization and protein kinase C (PKC) activation. It is expressed in various tissues, including the gastrointestinal tract, immune cells, and the central nervous system.
P2RY4 antibodies are essential tools for studying receptor localization, expression levels, and functional roles in physiological and pathological contexts. They are widely used in techniques such as Western blotting, immunohistochemistry, and flow cytometry. Research highlights P2Y4's involvement in processes like epithelial secretion, immune response modulation, and neuroinflammation. Dysregulation of P2Y4 signaling has been implicated in diseases such as cystic fibrosis, inflammatory bowel disease, and certain cancers. Validated P2RY4 antibodies enable precise detection, aiding in mechanistic studies and potential therapeutic targeting. However, specificity validation (e.g., via knockout controls) is crucial due to structural similarities among P2Y receptor subtypes. Ongoing research continues to explore P2Y4's pathophysiological relevance, positioning P2RY4 antibodies as key reagents in purinergic signaling research.
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