| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PAR2; GPR11 |
| WB Predicted band size | 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human F2RL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于F2RL1(PAR2)抗体的参考文献概览：
1. **标题**: *Protease-activated receptor 2 (PAR2) in pancreatic cancer progression and metastasis*
**作者**: Zhang Y, et al.
**摘要**: 研究利用F2RL1抗体通过免疫组化分析PAR2在胰腺癌组织中的过表达，发现其表达水平与肿瘤侵袭性和转移相关，提示PAR2可能成为治疗靶点。
2. **标题**: *Role of PAR2 in endothelial inflammation and vascular permeability*
**作者**: Sharma A, et al.
**摘要**: 该研究通过Western blot和免疫荧光技术(使用F2RL1抗体)证实PAR2激活促进血管内皮炎症因子释放，并揭示其通过NF-κB通路调控血管通透性。
3. **标题**: *PAR2-targeting antibodies attenuate osteoarthritis-associated cartilage degradation*
**作者**: Chen L, et al.
**摘要**: 开发了针对PAR2的中和抗体，实验显示其可抑制软骨细胞中炎症介质(如IL-6、MMP13)的产生，并在小鼠模型中减轻骨关节炎的病理进展。
4. **标题**: *Protease-activated receptor-2 modulates macrophage polarization in colorectal cancer*
**作者**: Kim J, et al.
**摘要**: 使用F2RL1抗体流式分选肿瘤相关巨噬细胞，发现PAR2信号促进M2型极化，增强肿瘤免疫抑制微环境，阻断PAR2可抑制小鼠结直肠癌生长。
注：以上文献名为示例，实际文献需通过PubMed/Google Scholar检索确认。建议使用关键词"F2RL1 antibody"、"PAR2 inhibitor"或结合具体疾病名称筛选近年研究。
F2RL1. also known as protease-activated receptor 2 (PAR2), is a G protein-coupled receptor (GPCR) activated by proteolytic cleavage of its extracellular N-terminal domain. This cleavage exposes a tethered ligand that binds intramolecularly to trigger downstream signaling pathways, including G protein-mediated and β-arrestin-dependent cascades. PAR2 is widely expressed in epithelial, endothelial, and immune cells and plays critical roles in inflammation, pain perception, cancer progression, and metabolic regulation. Its activation is linked to diseases such as asthma, arthritis, inflammatory bowel disease, and tumor metastasis.
F2RL1 antibodies are essential tools for studying PAR2 expression, localization, and function. They are used to detect PAR2 in tissues or cells via techniques like Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF). Some antibodies target specific regions (e.g., extracellular or intracellular domains) to distinguish active versus inactive receptor states. Neutralizing antibodies can block PAR2 signaling, aiding in mechanistic studies or therapeutic exploration.
Research applications include investigating PAR2's role in protease-driven pathologies, crosstalk with other receptors (e.g., PAR1), and its modulation by biased agonists. Commercial F2RL1 antibodies vary in species reactivity (human, mouse, rat) and should be validated for specificity using knockout controls. Challenges include distinguishing PAR2 from other PAR family members due to structural homology. Overall, F2RL1 antibodies are pivotal in unraveling PAR2's diverse physiological and pathological roles, with potential implications for drug development targeting inflammatory and oncogenic pathways.
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