| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | Nbla00170 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human NES |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NES(Nestin)抗体的3篇代表性文献示例(注：文献信息为示例性概括，非真实文献)：
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1. **文献名称**: "Nestin expression in embryonic and adult human neural progenitor cells"
**作者**: Lendahl U, Zimmerman LB, McKay RD
**摘要**: 该研究首次报道Nestin(NES)作为神经上皮干细胞特异性中间丝蛋白的标记物，发现其在胚胎和成体神经祖细胞中高表达，并参与神经系统的发育和再生过程。
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2. **文献名称**: "Comparative analysis of nestin in mammalian CNS development"
**作者**: Dahlstrand J, Lardelli M, Lendahl U
**摘要**: 通过比较不同哺乳动物中枢神经系统，揭示了NES抗体在识别神经前体细胞中的保守性，并探讨其表达动态与神经分化阶段的相关性。
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3. **文献名称**: "Nestin as a biomarker for stem cells and cancer stem cells"
**作者**: Wiese C, Rolletschek A, Kania G et al.
**摘要**: 综述NES抗体在干细胞研究中的应用，强调其在肿瘤干细胞(如胶质母细胞瘤)检测中的价值，并讨论其作为病理诊断标志物的潜力。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词(如"Nestin antibody"、"NES marker")获取近年研究。
The nuclear export signal (NES) is a short amino acid sequence that mediates the transport of proteins from the nucleus to the cytoplasm via the CRM1 (chromosome region maintenance 1)-dependent export pathway. NES-containing proteins play critical roles in regulating cellular processes such as gene expression, cell cycle progression, and stress responses. Antibodies targeting NES sequences or NES-bearing proteins are essential tools in studying nucleocytoplasmic shuttling dynamics, protein localization, and functional interactions within this transport system.
NES antibodies are widely used in immunofluorescence, Western blotting, and immunoprecipitation to detect proteins with functional NES motifs, such as tumor suppressors, viral proteins, or signaling molecules. For example, they help investigate the nuclear export of p53. NF-κB, or HIV Rev protein, providing insights into disease mechanisms like cancer or viral infection. These antibodies also aid in validating the efficacy of CRM1 inhibitors (e.g., leptomycin B or selinexor), which block NES-dependent export and are explored as therapies for leukemia or solid tumors.
However, NES antibody specificity can vary due to sequence diversity and structural flexibility of NES motifs. Researchers must validate results with complementary techniques, such as mutagenesis or live-cell imaging, to confirm functional NES activity. Despite challenges, these antibodies remain pivotal in dissecting the spatial regulation of proteins and their pathological implications.
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