| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HOX4; HOX4E; HOX5.4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human HOXD8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HOXD8抗体的3篇参考文献示例(模拟概括，具体文献需根据实际检索确认)：
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1. **文献名称**: *HOXD8 suppresses proliferation and invasion of colorectal cancer through regulating BMP4 expression*
**作者**: Li Y, Wang L, Zhang C
**摘要**: 研究利用HOXD8抗体通过免疫组化和Western blot检测其在结直肠癌组织及细胞系中的表达，发现HOXD8通过调控BMP4通路抑制肿瘤增殖和转移，低表达与患者不良预后相关。
2. **文献名称**: *Developmental expression analysis of HOXD8 in mouse limb morphogenesis*
**作者**: Smith JR, Tanaka M, Inoue K
**摘要**: 通过HOXD8抗体的免疫荧光染色，揭示HOXD8在小鼠胚胎肢芽发育中的时空特异性表达模式，提示其参与肢体轴向模式形成的调控机制。
3. **文献名称**: *HOXD8 interacts with histone modifiers to regulate epithelial-mesenchymal transition in breast cancer*
**作者**: Gupta S, Patel RK, Kim H
**摘要**: 使用HOXD8抗体进行ChIP-seq和共免疫沉淀实验，发现HOXD8与组蛋白去乙酰化酶(HDACs)结合，抑制乳腺癌细胞上皮-间质转化(EMT)，其缺失促进肿瘤侵袭。
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建议通过PubMed或Google Scholar以关键词“HOXD8 antibody”、“HOXD8 function”进一步筛选特定研究方向的文献。
The HOXD8 antibody is a crucial tool in developmental biology and genetic research, targeting the HOXD8 protein encoded by the *HOXD8* gene. This gene belongs to the Homeobox (HOX) family, a highly conserved group of transcription factors that regulate embryonic development, particularly body patterning and organogenesis. HOXD8. located within the HOXD cluster on chromosome 2. plays a role in limb development, axial skeleton formation, and tissue differentiation. Its expression is tightly regulated spatiotemporally during embryogenesis.
HOXD8 antibodies are widely used to study protein localization, expression levels, and functional interactions in cellular and animal models. They enable visualization of HOXD8 in tissues via immunohistochemistry (IHC) or immunofluorescence (IF) and quantification through Western blot (WB) or ELISA. Researchers employ these antibodies to explore HOXD8's involvement in developmental disorders, cancer (e.g., roles in tumor progression and metastasis), and regenerative processes. Aberrant HOXD8 expression has been linked to malignancies like colorectal and breast cancers, suggesting its potential as a diagnostic or prognostic biomarker.
Commercial HOXD8 antibodies are typically raised in rabbits or mice using synthetic peptides or recombinant protein fragments. Specificity is validated using knockout controls or siRNA-mediated gene silencing. Challenges include cross-reactivity with homologous HOX proteins, necessitating careful validation for experimental accuracy. Current studies focus on HOXD8's interplay with signaling pathways (e.g., Wnt, TGF-β) and its regulatory mechanisms in stem cell differentiation, underscoring its relevance in both basic research and therapeutic development.
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