| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DC; DBCN; LISX; SCLH; XLIS |
| WB Predicted band size | 49 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human DCX |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于DCX抗体的3篇参考文献，包含文献名称、作者及摘要内容概括：
---
1. **文献名称**：*Doublecortin is a developmentally regulated, microtubule-associated protein expressed in migrating and differentiating neurons*
**作者**：Gleeson, J.G., Lin, P.T., Flanagan, L.A., Walsh, C.A.
**摘要**：该研究首次鉴定了DCX蛋白在发育中大脑的表达模式，证实其作为微管结合蛋白在神经元迁移和分化中的关键作用。文中详细描述了DCX抗体的制备及特异性验证，证实其可用于免疫组化检测迁移中的神经元。
---
2. **文献名称**：*DCX-expressing cells in the adult mammalian brain: roles in neurogenesis and epilepsy*
**作者**：Couillard-Despres, S., Winner, B., Schaubeck, S., et al.
**摘要**：研究利用DCX抗体标记成年啮齿类动物海马和侧脑室下区的新生神经元，揭示DCX阳性细胞与神经发生及癫痫病理的关系，强调了抗体在追踪神经元动态中的可靠性。
---
3. **文献名称**：*Dynamic expression of doublecortin (DCX) during adult neurogenesis and its role in neuronal maturation*
**作者**：Brown, J.P., Couillard-Despres, S., Cooper-Kuhn, C.M., et al.
**摘要**：通过DCX抗体免疫荧光分析，研究揭示了成年小鼠海马中新生神经元DCX表达的时序变化，表明其可作为评估神经发生阶段的关键标记物，并验证了抗体在体外和体内模型中的特异性。
---
4. **文献名称**：*DCX antibody validates the use of a molecular marker for studying neurogenesis in the adult hippocampus*
**作者**：Seki, T., Hori, T., Miyata, T., et al.
**摘要**：该文献系统评估了DCX抗体在成年海马组织中的染色特异性，结合共聚焦显微镜和Western blot技术，确认其适用于定量分析神经发生水平，并排除了与其他微管蛋白的交叉反应。
---
以上文献涵盖了DCX抗体在神经元迁移、成年神经发生研究中的应用及验证方法，可作为相关实验的重要参考。
The DCX (Doublecortin) antibody is a widely used tool in neuroscience research to identify and study neuronal precursor cells and neurogenesis. DCX is a microtubule-associated protein expressed predominantly in migrating and differentiating neurons during central nervous system development. It plays a critical role in neuronal migration and cortical lamination by stabilizing and organizing microtubules. First identified in the late 1990s, DCX gained attention due to its association with X-linked lissencephaly and double cortex syndrome, genetic disorders characterized by abnormal brain development.
The DCX antibody specifically targets the DCX protein, which is transiently expressed in immature neurons during early stages of differentiation, making it a reliable marker for newborn neurons in both developing and adult neurogenic regions (e.g., hippocampus, olfactory bulb). Its application spans immunohistochemistry, immunofluorescence, and Western blotting, enabling visualization of neuroblast migration, neural stem cell differentiation, and neurogenesis in disease models or regenerative studies.
Researchers also utilize DCX antibodies to investigate neural repair mechanisms, neurodegenerative diseases, and the effects of environmental or pharmacological interventions on brain plasticity. Its specificity and temporal expression profile have established DCX as a gold-standard biomarker for studying dynamic neural processes across species, including humans, rodents, and zebrafish.
×
