| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CAK; p34; p37; CycH |
| WB Predicted band size | 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human CCNH |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CCNH抗体的3-4篇参考文献的示例(部分为假设性文献，供参考)：
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1. **文献名称**: "Development and validation of a monoclonal antibody specific for human Cyclin H"
**作者**: Yan, Q., Liu, Y., & Chen, J.
**摘要**: 本研究报道了一种针对人源CCNH蛋白的高特异性单克隆抗体的开发，通过Western blot、免疫沉淀和免疫荧光验证其灵敏度和特异性。该抗体成功应用于CDK7-CCNH复合物的功能研究，为细胞周期调控机制提供了工具支持。
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2. **文献名称**: "Cyclin H overexpression correlates with poor prognosis in non-small cell lung cancer: an immunohistochemical study"
**作者**: Zhang, L., Wang, H., & Li, M.
**摘要**: 通过免疫组化技术(使用兔抗人CCNH多克隆抗体)，研究分析了肺癌组织中CCNH的表达水平，发现其高表达与患者总生存期缩短显著相关，提示CCNH可能作为肺癌预后的潜在生物标志物。
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3. **文献名称**: "Cyclin H is essential for transcriptional regulation and DNA damage repair via the CDK7 complex"
**作者**: Rochette, P.J., Drouin, R., & Therrien, J.P.
**摘要**: 利用CCNH抗体进行染色质免疫沉淀(ChIP)和蛋白质互作分析，揭示CCNH通过CDK7复合物调控RNA聚合酶II的磷酸化，并参与维持基因组稳定性。敲低CCNH导致DNA修复能力显著下降。
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4. **文献名称**: "Targeting Cyclin H: a novel therapeutic strategy for HIV-1 latency reversal"
**作者**: Parada, C.A., & Jeang, K.T.
**摘要**: 研究采用CCNH抗体阻断实验，证实CCNH在HIV-1 Tat蛋白介导的病毒转录激活中起关键作用。通过抑制CCNH-CDk7复合物活性，可有效逆转病毒潜伏状态，为抗病毒治疗提供新思路。
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**注**：以上文献为示例性质，实际引用时需核实数据库(如PubMed、Web of Science)中的真实文献信息。
CCNH antibody targets Cyclin H, a regulatory protein involved in transcriptional processes and cell cycle control. Cyclin H, encoded by the *CCNH* gene, forms a critical component of the CDK-activating kinase (CAK) complex alongside CDK7 and MAT1. This complex plays dual roles: it phosphorylates and activates CDKs (e.g., CDK1. CDK2. CDK4) to drive cell cycle progression and participates in transcription by regulating RNA polymerase II via phosphorylation of its C-terminal domain. Dysregulation of Cyclin H is linked to transcriptional defects, cell cycle arrest, and diseases such as cancer.
CCNH antibodies are widely used in research to study cell cycle dynamics, DNA repair mechanisms, and transcriptional regulation. They enable detection of Cyclin H expression levels in tissues or cells through techniques like Western blotting, immunohistochemistry, and immunofluorescence. Additionally, these antibodies aid in exploring CAK complex assembly and its interaction with the TFIIH transcription factor complex, which is implicated in nucleotide excision repair. Abnormal Cyclin H expression has been observed in certain cancers, making its antibody a tool for investigating oncogenic pathways or therapeutic targets. Studies also associate *CCNH* mutations with rare disorders like autosomal recessive hypotrichosis, highlighting its broader biomedical relevance.
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