关键信息
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基因名
CLIC5
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简介
The CLIC5 protein is essential for normal hearing and contributes to the formation of stereocilia and the development of the organ of Corti in the inner ear. It forms poorly selective ion channels that may transport chloride ions. CLIC5 Protein, Human (His) is the recombinant human-derived CLIC5 protein, expressed by E. coli , with N-6*His labeled tag.
- 应用
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别名
rHuChloride intracellular channel protein 5/CLIC5, His; Chloride Intracellular Channel Protein 5; CLIC5
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NZA1-2
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表达区间
M1-S251
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氨基酸序列
MTDSATANGDDRDPEIELFVKAGIDGESIGNCPFSQRLFMILWLKGVVFNVTTVDLKRKPADLHNLAPGTHPPFLTFNGDVKTDVNKIEEFLEETLTPEKYPKLAAKHRESNTAGIDIFSKFSAYIKNTKQQNNAALERGLTKALKKLDDYLNTPLPEEIDANTCGEDKGSRRKFLDGDELTLADCNLLPKLHVVKIVAKKYRNYDIPAEMTGLWRYLKNAYARDEFTNTCAADSEIELAYADVAKRLSRS
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蛋白长度
Full Length of Isoform-2
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分子量
37.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
CLIC5 (Chloride Intracellular Channels 5) is a member of the CLIC protein family, which consists of chloride ion channels involved in various cellular processes. The research background surrounding CLIC5 focuses on its role in ion transport, cellular signaling, and involvement in diseases such as cancer and cardiovascular disorders. Unlike other ion channels, CLIC proteins exist in both soluble and membrane-associated forms, which suggests their versatile roles in cellular physiology. Recent studies have indicated that CLIC5 is implicated in modulating cell migration, proliferation, and apoptosis, emphasizing its potential as a therapeutic target. Furthermore, CLIC5 has been shown to interact with various signaling pathways, including those linked to inflammatory responses and cytoskeletal dynamics. Understanding the structure and function of CLIC5, particularly through recombinant protein studies, can provide insights into its mechanisms of action and the consequences of its dysregulation in pathological conditions. As researchers continue to explore CLIC5's properties and interactions, the potential for developing CLIC5-modulating drugs or interventions becomes increasingly compelling, highlighting its relevance in both basic research and clinical applications.
