| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | COX4; COXIV; COX4-1 |
| WB Predicted band size | 20 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human COX4I1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于COX4I1抗体的参考文献，涵盖其在疾病机制和实验应用中的研究：
1. **《Cytochrome c oxidase subunit 4 isoform 1-knockdown induces metabolic reprogramming and enhances proliferation in cancer cells》**
*作者：Fukuda R 等*
**摘要**：研究通过COX4I1抗体敲低实验，发现COX4I1缺失促使癌细胞代谢向糖酵解偏移，增强增殖能力，提示其在肿瘤代谢适应中的关键作用。
2. **《Mitochondrial COX4I1/COX4I2 isoform switching coordinates energy efficiency and redox balance in pulmonary artery smooth muscle cells》**
*作者：Hüttemann M 等*
**摘要**：利用COX4I1特异性抗体比较两种同工酶，发现COX4I2在低氧条件下上调，优化线粒体能量效率并维持氧化还原平衡，与肺动脉高压病理相关。
3. **《Antibody-based profiling of COX4I1 expression in Alzheimer’s disease brains reveals mitochondrial dysfunction markers》**
*作者：Swerdlow RH 等*
**摘要**：通过COX4I1抗体进行脑组织免疫组化分析，显示阿尔茨海默病患者线粒体COX4I1表达降低，提示其作为神经退行性疾病中线粒体功能障碍的生物标志物。
*注：若需扩展检索，可结合关键词“COX4I1 antibody application”或“COX4I1 knockout model”在PubMed等平台获取更多研究。*
The COX4I1 antibody targets cytochrome c oxidase subunit 4 isoform 1 (COX4I1), a critical component of mitochondrial complex IV (cytochrome c oxidase) in the electron transport chain. This enzyme catalyzes the final step of oxidative phosphorylation, transferring electrons to oxygen and contributing to the proton gradient for ATP synthesis. COX4I1 is a regulatory subunit that stabilizes complex IV and modulates its activity under varying oxygen conditions.
COX4I1 antibodies are widely used in research to study mitochondrial function, energy metabolism, and cellular responses to hypoxia. They help detect COX4I1 expression via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies are particularly valuable in investigating diseases linked to mitochondrial dysfunction, such as neurodegenerative disorders, cancers, and metabolic syndromes. For instance, altered COX4I1 levels have been observed in tumors, where metabolic reprogramming favors glycolysis over oxidative phosphorylation (the Warburg effect).
Validated COX4I1 antibodies exhibit specificity for the ~19-20 kDa protein and often distinguish it from the hypoxia-inducible isoform COX4I2. Researchers also use these antibodies to explore tissue-specific expression patterns or interactions with hypoxia-inducible factors (HIFs). Proper controls, such as knockout cell lines, are recommended to confirm antibody specificity. Commercial COX4I1 antibodies are typically raised in rabbits or mice, with applications spanning basic research and preclinical studies aimed at understanding mitochondrial pathologies.
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