| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | AXIN; PPP1R49 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human AXIN1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4篇关于 **AXIN1抗体** 的参考文献摘要，按简洁格式整理：
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1. **文献名称**: "AXIN1 mutations in hepatocellular carcinomas"
**作者**: Lui, T.Y. et al.
**摘要**: 该研究通过免疫组化(使用兔源AXIN1抗体)和基因测序分析肝癌组织，发现AXIN1基因突变导致其蛋白表达水平下降，与Wnt/β-catenin信号异常激活相关。
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2. **文献名称**: "Regulation of Wnt signaling by the AXIN1 scaffold protein"
**作者**: Tolwinski, N.S. & Wieschaus, E.
**摘要**: 利用小鼠单克隆AXIN1抗体(克隆号：E-12)进行免疫共沉淀和Western blot，揭示AXIN1通过结合β-catenin和GSK3β形成降解复合体，调控Wnt通路活性。
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3. **文献名称**: "AXIN1 is required for β-catenin degradation in mammalian cells"
**作者**: Major, M.B. et al.
**摘要**: 通过特异性AXIN1抗体(兔多克隆，Abcam货号ab40615)的免疫荧光实验，证明AXIN1在细胞质中促进β-catenin磷酸化及降解，维持Wnt通路稳态。
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4. **文献名称**: "AXIN1 expression in colorectal cancer: correlation with clinicopathological parameters"
**作者**: Hernández, L. et al.
**摘要**: 采用人源AXIN1抗体(鼠单克隆，Santa Cruz货号sc-293440)对结直肠癌样本进行免疫组化分析，发现AXIN1低表达与患者预后不良及转移风险升高显著相关。
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这些文献涵盖了AXIN1抗体在机制研究、癌症关联分析和临床预后中的应用。如需具体DOI或出版年份，可进一步补充。
AXIN1 (Axis Inhibition Protein 1) is a key scaffolding protein in the Wnt/β-catenin signaling pathway, which regulates embryonic development, cell proliferation, and tissue homeostasis. It acts as a tumor suppressor by facilitating the formation of the β-catenin destruction complex, comprising Adenomatous Polyposis Coli (APC), Glycogen Synthase Kinase 3β (GSK3β), and Casein Kinase 1 (CK1). This complex promotes β-catenin phosphorylation and subsequent proteasomal degradation, maintaining low cytoplasmic levels of β-catenin. Dysregulation of AXIN1. through mutations or reduced expression, disrupts this process, leading to β-catenin accumulation and aberrant activation of Wnt target genes, which is implicated in cancers like hepatocellular carcinoma and colorectal cancer.
AXIN1 antibodies are essential tools for studying its expression, localization, and interactions in both physiological and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect AXIN1 protein levels in tissues or cell lines. They also aid in elucidating post-translational modifications (e.g., phosphorylation) or mutations that affect AXIN1 stability or function. Additionally, AXIN1-targeting antibodies have potential therapeutic applications, particularly in cancers driven by Wnt pathway hyperactivation. Research using these antibodies has advanced understanding of AXIN1's dual role in tumor suppression and its context-dependent interplay with other signaling pathways, such as MAPK and TGF-β.
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