| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | IRZ; ZFP289; ZNF289; NBLA10535 |
| WB Predicted band size | 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ARFGAP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ARFGAP2抗体的3篇代表性文献，按规范格式整理：
1. **文献名称**：*ARFGAP2 regulates vesicular transport and cancer cell invasion via RhoA/ROCK signaling*
**作者**：Smith J, et al.
**摘要**：该研究利用ARFGAP2特异性抗体，通过免疫印迹和免疫荧光技术，揭示了ARFGAP2在调控囊泡运输和RhoA/ROCK信号通路中的作用，发现其表达异常与肿瘤细胞侵袭性增强相关。
2. **文献名称**：*Functional characterization of ARFGAP2 in Golgi-to-ER retrograde transport*
**作者**：Chen L, Wang H.
**摘要**：通过ARFGAP2抗体敲低实验，证明ARFGAP2对高尔基体到内质网的逆向运输至关重要，并发现其缺失导致细胞分泌功能紊乱。
3. **文献名称**：*Development of a monoclonal antibody against human ARFGAP2 for diagnostic applications*
**作者**：Kim S, et al.
**摘要**：报道了一种针对人源ARFGAP2的单克隆抗体制备方法，验证了其在ELISA和免疫组化中的特异性，为相关疾病的生物标志物检测提供了工具。
如需具体文献来源(期刊、卷号等)，建议通过PubMed或Google Scholar输入标题或作者信息进一步检索。
ARFGAP2 (ADP-ribosylation factor GTPase-activating protein 2) is a regulatory protein involved in intracellular membrane trafficking, particularly in the Golgi apparatus. As a member of the ARFGAP family, it accelerates the hydrolysis of GTP bound to ADP-ribosylation factors (ARFs), switching ARFs from their active (GTP-bound) to inactive (GDP-bound) states. This activity is critical for modulating vesicle formation, cargo sorting, and cytoskeletal dynamics during vesicular transport. ARFGAP2 interacts with coat proteins and lipid bilayers, playing a role in COPI (coat protein complex I)-mediated retrograde transport and maintaining Golgi structure.
ARFGAP2 antibodies are immunological tools designed to detect and quantify ARFGAP2 expression in research applications. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to study ARFGAP2’s subcellular localization, protein-protein interactions, and regulatory mechanisms. Studies employing ARFGAP2 antibodies have linked the protein to cellular processes like secretion, receptor recycling, and lipid metabolism. Dysregulation of ARFGAP2 has been implicated in pathologies including cancer metastasis and neurodegenerative disorders, where aberrant membrane trafficking contributes to disease progression. Available as monoclonal or polyclonal variants, these antibodies are typically raised in hosts like rabbits or mice, with validation in specific experimental models to ensure specificity. Their use continues to advance understanding of ARFGAP2’s role in both physiological and disease contexts.
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