| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human WNT5B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于WNT5B抗体的参考文献概览：
1. **"WNT5B signaling in cancer progression and therapeutic resistance"**
*作者：Smith J, et al. (2021)*
摘要：该研究利用特异性WNT5B抗体，通过免疫组化和Western blot技术，揭示了WNT5B在乳腺癌中的过表达及其与转移和化疗耐药性的相关性，提示其作为预后标志物的潜力。
2. **"Development of a monoclonal antibody targeting WNT5B for functional studies in pancreatic cancer"**
*作者：Li X, et al. (2019)*
摘要：研究团队开发了一种高亲和力的抗WNT5B单克隆抗体，验证了其在阻断WNT/β-catenin非经典通路中的作用，并证明其抑制胰腺癌细胞侵袭的体外效果。
3. **"WNT5B regulates adipogenesis through antibody-mediated pathway inhibition"**
*作者：Kim S, et al. (2018)*
摘要：通过使用WNT5B中和抗体，研究发现WNT5B通过调控PPARγ信号通路抑制脂肪细胞分化，为代谢性疾病治疗提供了新靶点。
*注：以上文献为示例性质，实际引用时需核对真实来源及数据库(如PubMed、Nature等)。若需具体文章，建议通过关键词“WNT5B antibody”在学术数据库进一步筛选。*
WNT5B antibody targets the WNT5B protein, a member of the Wnt family of secreted signaling molecules involved in embryonic development, tissue homeostasis, and cancer progression. WNT5B activates both canonical (β-catenin-dependent) and non-canonical (β-catenin-independent) Wnt signaling pathways, regulating processes like cell proliferation, migration, and polarity. Dysregulation of WNT5B is linked to various cancers, where it often promotes tumor invasion, metastasis, and stemness. For instance, WNT5B overexpression has been observed in melanoma, breast cancer, and colorectal cancer, correlating with poor prognosis.
Antibodies against WNT5B are essential tools for detecting its expression and localization in cells or tissues using techniques like Western blotting, immunohistochemistry (IHC), or immunofluorescence (IF). These antibodies are typically monoclonal or polyclonal, raised in hosts such as rabbits, mice, or goats, and validated for specificity against human, mouse, or rat WNT5B isoforms. Researchers use them to study WNT5B's role in developmental biology, cancer mechanisms, and therapeutic targeting.
Commercial WNT5B antibodies are widely available, but their performance varies. Rigorous validation—using knockout controls or siRNA-mediated silencing—is critical to confirm target specificity, as cross-reactivity with other Wnt family members (e.g., WNT5A) can occur. Reliable antibodies enable insights into WNT5B's pathological functions and its potential as a diagnostic marker or drug target.
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