| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human WNT8B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于WNT8B抗体的示例参考文献(注：以下内容为示例，实际文献需通过学术数据库查询)：
1. **"Characterization of WNT8B antibody specificity in vertebrate embryonic development"**
- Author: Smith A, et al.
- 摘要：该研究验证了一种多克隆WNT8B抗体在小鼠和斑马鱼模型中的特异性，发现其可用于检测胚胎发育过程中WNT8B蛋白的时空表达模式，并通过敲除实验确认抗体的信号依赖性。
2. **"WNT8B expression in human cancers: Validation of a novel monoclonal antibody"**
- Author: Lee H, et al.
- 摘要：开发并验证了一种高特异性抗人WNT8B的单克隆抗体，用于免疫组化分析，发现WNT8B在结直肠癌和胶质瘤中异常高表达，且与患者预后相关。
3. **"Functional analysis of WNT8B signaling using a blocking antibody in neural crest development"**
- Author: Garcia-Ruiz S, et al.
- 摘要：研究利用中和性WNT8B抗体阻断鸡胚神经嵴细胞的WNT信号通路，证明WNT8B在神经嵴迁移和分化中的关键作用，抗体特异性通过Western blot和免疫荧光验证。
4. **"A comparative study of commercial WNT8B antibodies for cross-species reactivity"**
- Author: Johnson R, et al.
- 摘要：评估了多种市售WNT8B抗体在人类、小鼠和非洲爪蟾中的交叉反应性，发现部分抗体存在非特异性结合，推荐特定抗体用于不同物种研究。
**建议**：实际文献请通过PubMed、Google Scholar或抗体供应商(如Abcam、CST)官网的引用文献板块查询，关键词“WNT8B antibody validation”或“WNT8B immunohistochemistry”。
The Wnt8b antibody is a crucial tool in studying the Wnt signaling pathway, which plays a pivotal role in embryonic development, tissue homeostasis, and disease. WNT8B, a member of the Wnt protein family, is a secreted glycoprotein involved in the Wnt/β-catenin signaling cascade. It regulates cell proliferation, differentiation, and polarity by binding to Frizzled receptors and co-receptors like LRP5/6. WNT8B is particularly significant in neural patterning, eye development, and skeletal morphogenesis. Dysregulation of WNT8B has been linked to developmental disorders and cancers, including neuroblastoma and endocrine tumors.
The Wnt8b antibody is designed to detect and quantify WNT8B protein expression in various experimental models, such as immunohistochemistry, Western blotting, and immunofluorescence. Researchers use it to investigate spatial-temporal expression patterns during embryogenesis, tissue regeneration, or disease progression. Commercially available antibodies are typically raised in rabbits or mice, targeting specific epitopes within the N-terminal or C-terminal regions of the WNT8B protein. Validation often includes knockout cell lines or tissues to confirm specificity.
Studies employing Wnt8b antibodies have advanced our understanding of its role in brain development, particularly in the midbrain-hindbrain boundary and retinal organization. In cancer research, it helps explore WNT8B's oncogenic potential through aberrant pathway activation. However, challenges remain, including cross-reactivity with homologous Wnt family members and variability in post-translational modifications. Proper controls and validation are essential for reliable data interpretation. This antibody remains indispensable for dissecting Wnt8B's functional mechanisms in developmental biology and disease pathology.
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