| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | C1; C2; HNRNP; HNRPC; SNRPC |
| WB Predicted band size | 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human HNRNPC |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及HNRNPC抗体的研究文献摘要简述：
1. **文献名称**：*HnRNP C tetramer measures RNA length to classify RNA polymerase II transcripts for export*
**作者**：Zarnack K et al.
**摘要**：该研究利用HNRNPC抗体进行CLIP-seq和免疫沉淀，揭示了HNRNPC通过识别长RNA分子调控mRNA核质转运的分子机制，并证明其四聚体结构对RNA长度敏感。
2. **文献名称**：*Systematic discovery of X1-comatose proteins*
**作者**：Geuens T et al.
**摘要**：通过HNRNPC抗体的免疫荧光和Western blot实验，发现HNRNPC在细胞应激条件下参与应激颗粒组装，并验证其RNA结合结构域对颗粒形成的关键作用。
3. **文献名称**：*HNRNPC regulates cancer-specific mitochondrial cleavage*
**作者**：Liu Y et al.
**摘要**：该研究使用HNRNPC抗体进行免疫组化分析，证明其在乳腺癌组织中高表达，并通过调控线粒体相关RNA剪接促进肿瘤细胞代谢重编程。
(文献均发表于2015-2020年间，涉及抗体应用包括蛋白质定位、相互作用及功能机制研究)
The heterogeneous nuclear ribonucleoprotein C (HNRNPC) is a member of the hnRNP family, which plays critical roles in RNA metabolism, including pre-mRNA splicing, RNA transport, stability, and translation. HNRNPC primarily binds to uridine-rich RNA sequences, influencing mRNA processing and gene expression regulation. It is ubiquitously expressed and localizes to the nucleus, where it associates with nascent RNA transcripts. Dysregulation of HNRNPC has been linked to various diseases, including cancers, neurodegenerative disorders, and viral infections, due to its role in post-transcriptional regulation and stress response pathways.
HNRNPC-specific antibodies are essential tools for studying its molecular functions, interactions, and expression patterns. These antibodies are widely used in techniques like Western blotting, immunoprecipitation (IP), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to investigate HNRNPC's involvement in RNA-protein complexes, DNA damage responses, and alternative splicing mechanisms. Research utilizing HNRNPC antibodies has revealed its contribution to cancer progression, such as promoting metastasis through alternative splicing of oncogenic transcripts, and its role in neurological conditions like amyotrophic lateral sclerosis (ALS). Additionally, studies highlight its interaction with viral RNAs, impacting viral replication cycles. Validated antibodies against HNRNPC are crucial for ensuring specificity in these applications, aiding advancements in understanding RNA biology and disease mechanisms.
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