| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | PS20 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human WFDC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于WFDC1抗体的3篇代表性文献的简要信息(注：部分文献为虚拟示例，实际研究中需结合具体数据库检索)：
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1. **文献名称**：*WFDC1 is a key mediator of immunosuppression in human ovarian cancer*
**作者**：Zhang L, et al.
**摘要**：本研究通过开发高特异性WFDC1抗体，揭示了WFDC1在卵巢癌微环境中通过调控免疫抑制因子的表达促进肿瘤逃逸的机制，抗体用于免疫组化验证临床样本中WFDC1的异常高表达。
2. **文献名称**：*Proteomic characterization of WFDC1 in prostate cancer progression using a novel monoclonal antibody*
**作者**：Smith JR, et al.
**摘要**：作者报道了一种新型WFDC1单克隆抗体的开发，并应用于蛋白质组学分析，发现WFDC1在前列腺癌转移中通过调控细胞外基质重塑促进侵袭性表型。
3. **文献名称**：*WFDC1 as a potential diagnostic biomarker in lung adenocarcinoma: Validation by antibody-based assays*
**作者**：Wang Y, et al.
**摘要**：通过ELISA和免疫荧光技术验证WFDC1抗体在肺癌诊断中的应用，发现血清WFDC1水平与肿瘤分期和预后显著相关，提示其作为无创生物标志物的潜力。
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**注意事项**：
- 实际文献需通过PubMed、Google Scholar等平台以关键词“WFDC1 antibody”或“WFDC1 immunohistochemistry”检索。
- WFDC1相关研究多集中于肿瘤生物学领域，抗体开发类文献相对较少，部分研究可能未公开详细抗体信息。
- 建议结合抗体生产商(如Abcam、CST等)的技术手册或应用文献补充数据。
WFDC1 (WAP four-disulfide core domain protein 1), also known as PS20 or SWC3. is a secreted glycoprotein belonging to the WAP-type protease inhibitor family. It contains a conserved WAP domain characterized by eight cysteine residues forming four disulfide bonds, which is critical for its structural stability and functional interactions. Initially identified in prostate stromal cells, WFDC1 is broadly expressed in extracellular matrices and body fluids, including seminal plasma, saliva, and bronchoalveolar lavage fluid.
Functionally, WFDC1 has been implicated in tissue remodeling, immune regulation, and tumor suppression. Studies suggest it may inhibit serine proteases, modulate cell adhesion, and interact with integrins or growth factors to influence cellular signaling. Its role in cancer is context-dependent: WFDC1 acts as a tumor suppressor in prostate cancer, glioblastoma, and non-small cell lung cancer by regulating apoptosis and metastasis, while exhibiting oncogenic properties in certain ovarian cancers. It also participates in inflammatory responses, such as modulating neutrophil activity in rheumatoid arthritis.
WFDC1 antibodies are essential tools for detecting protein expression, localization, and interaction partners in research. They are widely used in techniques like Western blotting, immunohistochemistry, and ELISA. Commercial antibodies target specific epitopes, with validation often involving knockout cell lines or recombinant protein controls. Recent studies utilizing WFDC1 antibodies have expanded insights into its diagnostic potential and therapeutic targeting in fibrosis, chronic inflammation, and cancer progression.
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