| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | BBIS; WDR58; fSAP35 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human THOC6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是模拟生成的关于THOC6抗体的参考文献示例(注：实际文献可能需要通过数据库进一步验证)：
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1. **文献名称**: "THOC6 regulates neuronal differentiation via mRNA export in neural progenitor cells"
**作者**: Smith A, et al.
**摘要**: 本研究利用THOC6特异性抗体，通过免疫沉淀和RNA测序分析，揭示了THOC6在小鼠神经祖细胞分化过程中通过选择性mRNA输出调控神经元基因表达的功能。抗体验证显示THOC6在细胞核内富集，敲低后导致分化阻滞。
2. **文献名称**: "A novel THOC6 mutation linked to X-linked intellectual disability: Functional characterization using antibody-based assays"
**作者**: Chen L, et al.
**摘要**: 作者发现了一个与X连锁智力障碍相关的THOC6新突变，并通过Western blot和免疫荧光(使用THOC6多克隆抗体)证明该突变导致蛋白稳定性下降及细胞质定位异常，提示其与疾病病理的相关性。
3. **文献名称**: "Interaction mapping of the THO complex subunits by co-immunoprecipitation"
**作者**: Gonzalez-Sanchez MB, et al.
**摘要**: 该研究使用针对THOC6及其他THO复合体组分的抗体进行共免疫沉淀实验，系统解析了复合体的相互作用网络，证实THOC6是维持复合体结构完整性的关键蛋白。
4. **文献名称**: "Commercial antibody validation for THOC6 in human tissues: A comparative study"
**作者**: Patel R, et al.
**摘要**: 评估了市售三种THOC6抗体在人类组织样本中的特异性，通过敲除细胞系验证抗体可靠性，并提供了适用实验条件(如IHC、WB)，为后续研究提供参考。
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**注意**：以上文献为示例性质，实际引用请通过PubMed、Web of Science等数据库检索关键词(如"THOC6 antibody"、"THOC6 function")获取真实文献。若需要具体文献协助，可提供更详细的研究方向以便进一步筛选。
The THOC6 antibody is a research tool used to study the THOC6 protein, a component of the THO complex, which is part of the larger TREX (Transcription-Export) complex involved in mRNA processing and nuclear export. THOC6 plays a critical role in coupling transcription with mRNA export, ensuring proper maturation and transport of mRNA from the nucleus to the cytoplasm. It is particularly important in developmental processes, as mutations in the *THOC6* gene are linked to neurodevelopmental disorders such as autosomal recessive intellectual disability (e.g., Alazami syndrome) and growth defects.
THOC6 antibodies are primarily used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect protein expression, localization, and interaction partners in various cell types or tissues. Studies utilizing these antibodies have revealed THOC6's tissue-specific roles, particularly in neural and embryonic tissues, and its interaction with other THO/TREX components (e.g., THOC2. THOC5). Research also explores how THOC6 dysfunction disrupts mRNA export, leading to cellular stress or developmental abnormalities.
The antibody’s specificity and validation (e.g., knockout controls) are critical, given the structural similarities among THO complex proteins. Ongoing work focuses on elucidating THOC6's molecular mechanisms in health and disease, highlighting its potential as a biomarker or therapeutic target in genetic disorders.
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