| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | HB58; PEP8A; VPS26; Hbeta58 |
| WB Predicted band size | 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human VPS26A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于VPS26A抗体的3篇参考文献，包含文献名称、作者及摘要概括：
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1. **文献名称**: *"Structure of the Mammalian Retromer Complex Reveals Selective Assembly and Architecture of SNX Coat"*
**作者**: Hierro, A., et al. (2007)
**摘要**: 本研究解析了哺乳动物retromer复合体的结构，其中VPS26A作为核心组分，通过X射线晶体学明确了其与VPS35的结合机制。研究中使用特异性VPS26A抗体进行免疫共沉淀实验，验证了复合体的组装及在细胞内运输中的作用。
2. **文献名称**: *"Cargo-selective Endosomal Sorting for Retrieval to the Golgi Requires Retromer"*
**作者**: Seaman, M.N. (2004)
**摘要**: 该研究揭示了retromer复合体(含VPS26A)在逆向运输中的选择性货物识别功能。通过VPS26A抗体的免疫荧光定位，证实其在内体膜上的富集，并证明其缺失导致溶酶体酶分选缺陷。
3. **文献名称**: *"Retromer Deficiency Observed in Alzheimer’s Disease Causes Hippocampal Neurodegeneration"*
**作者**: Small, S.A., & Gandy, S. (2006)
**摘要**: 研究探讨了阿尔茨海默病患者中retromer复合体(包括VPS26A)的表达下调及其与淀粉样蛋白病理的关系。采用VPS26A抗体的Western blot分析显示，患者脑组织中VPS26A蛋白水平显著降低，提示其与疾病进展相关。
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以上文献均直接应用或验证了VPS26A抗体，涵盖结构生物学、细胞运输机制及疾病机制研究。如需更多文献或特定实验细节，可进一步检索PubMed或抗体供应商的引用列表。
The VPS26A antibody is a crucial tool for studying the vacuolar protein sorting-associated protein 26A (VPS26A), a component of the retromer complex. This evolutionarily conserved complex facilitates retrograde transport of cargo proteins from endosomes to the trans-Golgi network, playing a vital role in intracellular trafficking, lysosomal degradation, and cellular signaling. VPS26A, along with VPS29 and VPS35. forms the retromer’s cargo-recognition subcomplex, essential for maintaining protein homeostasis. Dysregulation of VPS26A is linked to neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s) and neurodevelopmental disorders.
Researchers use VPS26A antibodies to investigate its expression, localization, and interactions in various cell types and tissues. These antibodies are critical for techniques like Western blotting, immunofluorescence, and immunoprecipitation, enabling the study of retromer dysfunction in disease models. Monoclonal and polyclonal variants are available, often validated for specificity using knockout controls. Recent studies also explore VPS26A’s role in autophagy, receptor recycling (e.g., Wnt signaling), and viral pathogenesis, highlighting its broad relevance. Commercial antibodies typically target epitopes within conserved regions, ensuring cross-species reactivity. Proper validation remains essential to avoid off-target effects, given structural similarities within the VPS protein family.
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