| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | SAFA; HNRPU; SAF-A; U21.1; pp120; EIEE54; GRIP120; hnRNP U; HNRNPU-AS1 |
| WB Predicted band size | 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human HNRNPU |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4篇关于HNRNPU抗体的参考文献及其摘要概括：
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1. **文献名称**：*HNRNPU mutations cause early-onset seizures and developmental delay*
**作者**：Nguyen et al. (2015)
**摘要**：研究通过全外显子测序发现HNRNPU基因突变与神经发育障碍相关，利用HNRNPU抗体进行蛋白质表达分析，揭示突变导致神经元细胞中HNRNPU功能异常，影响RNA剪接和基因表达调控。
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2. **文献名称**：*The role of HNRNPU in chromatin remodeling and transcriptional regulation*
**作者**：Damianov et al. (2018)
**摘要**：通过免疫沉淀(IP)和染色质结合实验(ChIP-seq)，结合HNRNPU抗体，证明HNRNPU与SWI/SNF染色质重塑复合体相互作用，调控胚胎干细胞分化和基因转录。
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3. **文献名称**：*HNRNPU promotes oncogenic RNA splicing in glioblastoma*
**作者**：Liang et al. (2020)
**摘要**：利用HNRNPU抗体进行Western blot和免疫荧光实验，发现HNRNPU在胶质母细胞瘤中高表达，通过异常RNA剪接驱动肿瘤增殖，敲低HNRNPU可抑制癌细胞生长。
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4. **文献名称**：*Functional characterization of HNRNPU variants in neurodevelopmental disorders*
**作者**：Holtgrewe et al. (2021)
**摘要**：通过CRISPR/Cas9构建HNRNPU突变细胞模型，结合抗体验证蛋白稳定性变化，发现致病突变导致HNRNPU核定位异常，破坏RNA代谢和突触功能。
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以上研究均通过HNRNPU抗体探索其在疾病机制中的分子功能，涵盖神经发育、癌症及表观遗传调控领域。
The heterogeneous nuclear ribonucleoprotein U (HNRNPU) antibody is a crucial tool in studying the functional roles of the HNRNPU protein, a member of the hnRNP family involved in RNA processing, chromatin remodeling, and genomic stability. HNRNPU binds to RNA and DNA, regulating mRNA splicing, transcriptional modulation, and maintaining nuclear architecture. It also plays a role in DNA repair and cell cycle control.
Antibodies targeting HNRNPU are widely used in molecular biology research to investigate its expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Dysregulation of HNRNPU is linked to neurodevelopmental disorders, including autosomal dominant intellectual disability, epilepsy, and developmental delays. Mutations in the HNRNPU gene are associated with syndromes such as early infantile epileptic encephalopathy, making its antibody valuable for mechanistic studies and disease modeling.
Commercial HNRNPU antibodies are typically raised against specific epitopes (e.g., human HNRNPU fragments) and validated for specificity across species (human, mouse, rat). Researchers use these antibodies to explore HNRNPU's role in cellular stress responses, neuronal development, and cancer biology, where aberrant RNA processing contributes to tumor progression. Proper validation using knockout controls ensures reliable results in both diagnostic and experimental contexts.
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