Rabbit Polyclonal UCP1 Antibody

UCP1 (Uncoupling Protein 1), also known as thermogenin, is a mitochondrial inner membrane protein predominantly expressed in brown adipose tissue (BAT). It plays a critical role in non-shivering thermogenesis by uncoupling oxidative phosphorylation from ATP production, thereby dissipating energy as heat. UCP1 antibodies are essential tools for studying BAT activity, metabolic regulation, and energy homeostasis. These antibodies enable the detection and quantification of UCP1 expression in tissues, cell cultures, or experimental models, aiding research on obesity, diabetes, and metabolic disorders. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess BAT activation, browning of white adipose tissue (WAT), or mitochondrial function. Due to UCP1's tissue-specific expression and structural similarities with other uncoupling proteins (e.g., UCP2. UCP3), antibody specificity is crucial to avoid cross-reactivity. Recent interest in UCP1 has surged with discoveries linking BAT activity to systemic metabolism and potential therapeutic strategies for weight management. However, challenges persist, including variable antibody performance across species and limited sensitivity in low-expression models. Validating UCP1 antibodies using knockout controls or siRNA-mediated knockdown remains critical for reliable data interpretation in studies exploring thermogenesis, adipocyte biology, or metabolic adaptations.