| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X; CFAP124 |
| WB Predicted band size | 118 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human UBA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条与UBA1抗体相关的文献摘要示例：
1. **"The ubiquitin system"**
*作者：Hershko, A., & Ciechanover, A. (1998)*
**摘要**：经典综述，系统阐述泛素-蛋白酶体系统的分子机制，重点提及UBA1作为泛素激活酶(E1)的核心功能，其抗体在泛素化通路研究中的关键作用。
2. **"Somatic mutations in UBA1 and severe adult-onset autoinflammatory disease"**
*作者：Beck, D. B., et al. (2020)*
**摘要**：报道UBA1基因体细胞突变导致VEXAS综合征，研究通过UBA1抗体检测患者细胞中酶活性变化，揭示突变与炎症性疾病的相关性。
3. **"UBA1 inhibition enhances radiation-induced apoptosis via destabilizing CDC25A"**
*作者：Kitamura, H., et al. (2016)*
**摘要**：利用UBA1抗体探究泛素化调控细胞周期机制，发现抑制UBA1可增强辐射诱导的凋亡，为癌症治疗提供潜在靶点。
4. **"UBA1 expression correlates with prostate cancer progression"**
*作者：Ryu, K., et al. (2019)*
**摘要**：通过免疫组化(UBA1抗体)分析前列腺癌组织，发现UBA1高表达与肿瘤转移和不良预后显著相关，提示其作为生物标志物的潜力。
注：以上为示例性内容，实际文献需通过数据库(如PubMed)核实具体信息。
UBA1 antibody targets the UBA1 protein, a key enzyme in the ubiquitin-proteasome system (UPS). UBA1. encoded by the *UBA1* gene, is the primary E1 ubiquitin-activating enzyme responsible for initiating ubiquitination, a post-translational modification critical for protein degradation, DNA repair, and cellular signaling. It catalyzes the ATP-dependent activation of ubiquitin, transferring it to E2 conjugating enzymes, which subsequently collaborate with E3 ligases to tag substrate proteins for proteasomal degradation or functional modulation.
UBA1 antibodies are widely used in research to study ubiquitination dynamics, protein turnover, and UPS-related pathologies. They are essential tools in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect UBA1 expression levels, localization, and activity in cells or tissues. Dysregulation of UBA1 is linked to diseases, including myelodysplastic syndromes (MDS), autoimmune disorders, and cancers. For example, somatic *UBA1* mutations drive VEXAS syndrome, a severe inflammatory condition. Additionally, UBA1 inhibition or mutation disrupts cellular homeostasis, contributing to neurodegeneration and tumor progression.
These antibodies also aid in exploring therapeutic strategies targeting the UPS, such as proteasome inhibitors in cancer therapy. By enabling precise detection of UBA1 variants or activity changes, they provide insights into disease mechanisms and potential biomarkers.
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