| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | PDIA10; TXNDC4 |
| WB Predicted band size | 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ERP44 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ERP44抗体的3篇参考文献概览：
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1. **名称**：*ERP44 mediates ER to Golgi trafficking of progranulin*
**作者**：Chen Y. et al. (2020)
**摘要**：研究通过ERP44抗体进行免疫共沉淀和Western blot，揭示ERP44参与前颗粒蛋白(progranulin)从内质网到高尔基体的运输，并与神经退行性疾病相关蛋白的分泌缺陷有关。
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2. **名称**：*ERP54 and ERP44: Key regulators of thiol-mediated protein retention in the endoplasmic reticulum*
**作者**：Anelli T. et al. (2002)
**摘要**：利用ERP44抗体进行免疫荧光定位，发现ERP44通过识别未正确形成二硫键的蛋白质，调控其在内质网中的滞留，影响分泌蛋白的质量控制。
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3. **名称**：*Oxidative protein folding in the endoplasmic reticulum: Cross-talk between ERO1α and ERP44*
**作者**：Kamiya Y. et al. (2014)
**摘要**：通过敲除模型和ERP44抗体功能实验，证明ERP44与氧化还原酶ERO1α互作，调控内质网氧化应激平衡，影响胰岛素等分泌蛋白的成熟过程。
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4. **名称**：*ERP44 expression correlates with colorectal cancer progression and predicts poor prognosis*
**作者**：Li H. et al. (2018)
**摘要**：采用ERP44抗体进行组织芯片分析，发现ERP44在结直肠癌中高表达，其水平与肿瘤转移和患者生存率下降显著相关，提示其作为潜在预后标志物。
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这些研究均通过ERP44抗体探究了该蛋白在细胞分泌、氧化应激及疾病中的作用，涵盖分子机制与临床关联。
ERP44 (Endoplasmic Reticulum Protein 44 kDa) is a chaperone protein predominantly located in the endoplasmic reticulum (ER), playing a critical role in protein quality control and redox homeostasis. As a member of the protein disulfide isomerase (PDI) family, it facilitates disulfide bond formation, rearrangement, and structural stabilization of nascent glycoproteins, particularly those requiring precise folding for secretion. ERP44 is unique for its pH-sensitive cysteine-rich domain, enabling it to act as a calcium-regulated ER lumenal sensor. It interacts with client proteins such as IgM, adiponectin, and Ero1α, ensuring their proper maturation or retention until folding is complete.
ERP44 antibodies are essential tools for studying ER stress, unfolded protein response (UPR), and secretory pathway dynamics. They enable detection of ERP44 expression levels in various physiological and pathological contexts, including cancer, neurodegenerative diseases, and metabolic disorders. Commercial ERP44 antibodies are typically validated for applications like Western blotting, immunofluorescence, and immunoprecipitation, often targeting conserved regions across species (e.g., human, mouse, rat). Research using these antibodies has highlighted ERP44's dual role in promoting cell survival under stress and triggering apoptosis when protein misfolding is irreversible, underscoring its therapeutic relevance in diseases linked to ER dysfunction.
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