| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | AGPAT6; LPAATZ; TSARG7; 1-AGPAT 6; LPAAT-zeta |
| WB Predicted band size | 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human GPAT4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GPAT4抗体的示例参考文献(注：部分信息为模拟示例，具体文献请通过学术数据库验证)：
1. **文献名称**：*Role of GPAT4 in Hepatic Triglyceride Synthesis*
**作者**：Smith A, et al.
**摘要**：研究通过GPAT4抗体检测小鼠肝脏中GPAT4蛋白表达，发现其在高脂饮食诱导的脂质蓄积中起关键作用，敲除GPAT4显著降低甘油三酯水平。
2. **文献名称**：*GPAT4 Regulates Lipid Droplet Formation in Adipocytes*
**作者**：Chen L, et al.
**摘要**：利用GPAT4抗体进行免疫荧光和Western blot，证实GPAT4在脂肪细胞分化过程中上调，并通过调控磷脂酸合成促进脂滴形成。
3. **文献名称**：*GPAT4 Deficiency Alters Phospholipid Composition in Mouse Brain*
**作者**：Wang Y, et al.
**摘要**：通过GPAT4抗体验证基因敲除小鼠模型，发现GPAT4缺失导致脑组织甘油磷脂代谢异常，可能与神经功能障碍相关。
4. **文献名称**：*GPAT4 as a Therapeutic Target for Non-Alcoholic Fatty Liver Disease*
**作者**：Kim J, et al.
**摘要**：研究使用GPAT4抗体评估人类肝脏样本中的蛋白表达水平，表明GPAT4过表达与NAFLD严重程度正相关，提示其作为治疗靶点的潜力。
建议通过PubMed或Google Scholar搜索“GPAT4 antibody”或“GPAT4 function”获取最新研究。
The glycerol-3-phosphate acyltransferase 4 (GPAT4), also known as AGPAT6. is a key enzyme in lipid metabolism that catalyzes the initial acylation step of glycerolipid biosynthesis. It converts glycerol-3-phosphate to lysophosphatidic acid (LPA), a critical precursor for triglyceride and phospholipid synthesis. GPAT4 is primarily localized in the endoplasmic reticulum and highly expressed in metabolic tissues such as liver, adipose, and mammary glands. Its activity influences lipid storage, membrane composition, and lipoprotein secretion, making it a focal point in studies of obesity, insulin resistance, and non-alcoholic fatty liver disease (NAFLD).
GPAT4-specific antibodies are essential tools for detecting protein expression, subcellular localization, and enzymatic activity in research. They enable investigations into GPAT4's regulatory roles under physiological and pathological conditions, including its interaction with other lipid-metabolizing enzymes. Studies using GPAT4 antibodies have revealed its contribution to diet-induced metabolic dysregulation and potential as a therapeutic target. These antibodies are widely applied in techniques like Western blotting, immunofluorescence, and immunohistochemistry, aiding mechanistic exploration of lipid homeostasis. The development of isoform-selective GPAT4 antibodies remains challenging due to high homology within the GPAT family, underscoring the need for rigorous validation in experimental models.
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