| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CAT2; ECAC1; OTRPC3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human TRPV5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TRPV5抗体的3篇参考文献(基于模拟数据整理，实际文献需通过学术数据库验证)：
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1. **标题**: *"A polyclonal antibody targeting TRPV5 reveals its apical localization in renal distal tubular cells"*
**作者**: Hoenderop JG, et al.
**摘要**: 本研究开发了一种针对TRPV5的多克隆抗体，通过免疫组化和Western blot验证其特异性，证实TRPV5在肾脏远曲小管顶膜的高表达，并揭示其在钙离子主动重吸收中的关键作用。
2. **标题**: *"Validation of a novel TRPV5 antibody for functional studies in calcium-transporting epithelia"*
**作者**: van der Eerden BC, et al.
**摘要**: 通过免疫荧光和siRNA敲除实验验证了一种高特异性TRPV5抗体，证明其在肠道和肾上皮细胞模型中的应用价值，并发现TRPV5表达受维生素D3调控。
3. **标题**: *"TRPV5 antibody-based profiling in hypercalciuric mouse models"*
**作者**: Takahashi N, et al.
**摘要**: 利用TRPV5特异性抗体分析高尿钙症小鼠模型，发现肾脏TRPV5蛋白水平显著降低，提示其表达异常可能与病理性钙流失相关。
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**备注**：以上文献为示例性质，实际引用时请通过PubMed、Web of Science等平台检索最新或经典文献，并确保作者、标题及摘要内容准确。若需具体文献，可提供数据库检索协助。
TRPV5 (Transient Receptor Potential Vanilloid 5) is a calcium-selective ion channel critical for maintaining systemic calcium homeostasis. Primarily expressed in the kidney, intestine, and placenta, it facilitates calcium reabsorption in the distal tubules of the nephron, playing a pivotal role in fine-tuning urinary calcium excretion. Dysregulation of TRPV5 has been implicated in hypercalciuria, kidney stone formation, and osteoporosis, making it a therapeutic target for calcium-related disorders.
TRPV5 antibodies are essential tools for studying the expression, localization, and function of this channel in physiological and pathological contexts. These antibodies are typically developed against specific epitopes of TRPV5. such as its intracellular N- or C-terminal regions, or extracellular loops. Validated through techniques like Western blotting, immunohistochemistry, and immunofluorescence, they help researchers detect TRPV5 protein levels in tissues or cultured cells.
In research, TRPV5 antibodies have been employed to investigate hormonal regulation (e.g., by vitamin D, parathyroid hormone) and interactions with calcium-binding proteins like calbindin-D28K. They also aid in exploring TRPV5's role in diseases, including renal calcium wasting syndromes and metabolic bone disorders. Specificity is confirmed using knockout models or siRNA-mediated knockdown. Both polyclonal and monoclonal TRPV5 antibodies are available, with selection depending on experimental needs. Their application continues to advance understanding of calcium transport mechanisms and potential therapeutic interventions.
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