| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | TIG1; CAG7A; CTG7A; PCQAP; TIG-1; TNRC7; ARC105 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human MED15 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与MED15抗体相关的研究文献摘要，供参考：
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1. **文献名称**：*MED15 modulates androgen receptor activation in prostate cancer cells*
**作者**：Mishra, V. et al.
**摘要**：该研究通过染色质免疫沉淀(ChIP)和免疫共沉淀(Co-IP)实验，利用MED15抗体揭示MED15作为中介体复合物亚基，在前列腺癌细胞中增强雄激素受体(AR)的转录活性，促进肿瘤生长。
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2. **文献名称**：*MED15 is required for adipocyte differentiation and lipid metabolism*
**作者**：Taubert, S. et al.
**摘要**：文章通过siRNA敲低MED15并结合Western blot(使用MED15抗体验证敲除效率)，证明MED15在脂肪细胞分化中调控PPARγ等关键基因，影响脂质代谢通路。
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3. **文献名称**：*MED15 interacts with TGF-β signaling to drive epithelial-mesenchymal transition*
**作者**：Zhang, L. et al.
**摘要**：研究利用MED15抗体进行免疫荧光和流式细胞术，发现MED15通过结合Smad蛋白增强TGF-β信号通路，促进癌细胞的上皮-间质转化(EMT)和转移。
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**备注**：如需具体文献来源(期刊、年份等)，建议通过PubMed或Google Scholar检索上述关键词获取全文信息。
The MED15 antibody is a research tool designed to detect MED15. a key subunit of the Mediator complex—a multi-protein assembly critical for transcriptional regulation in eukaryotes. The Mediator complex bridges transcription factors and RNA polymerase II, modulating gene expression. MED15. also known as PCQAP or ARC15. contains a conserved KID (kinase-inducible domain) that interacts with transcription factors like SMADs (in TGF-β signaling) and androgen receptor (AR), linking it to pathways involved in cancer progression, lipid metabolism, and stress responses.
MED15's role in diseases, particularly cancers (prostate, breast) and metabolic disorders (atherosclerosis), has driven interest in its study. Antibodies targeting MED15 enable researchers to investigate its expression, localization, and interactions via techniques like Western blotting, immunoprecipitation, or immunofluorescence. Commercial MED15 antibodies are typically raised in hosts such as rabbits or mice, with validation in human, mouse, or rat samples.
Studies using MED15 antibodies have revealed its overexpression in certain tumors and its regulatory effects on lipid homeostasis genes, suggesting therapeutic potential. However, antibody specificity remains a challenge due to Mediator complex heterogeneity. Researchers must validate batches using knockdown controls or epitope-tagged systems to ensure accurate detection. Overall, MED15 antibodies are pivotal for dissecting transcriptional mechanisms and disease pathways tied to this adaptable coactivator.
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