Rabbit Polyclonal ATG13 Antibody

ATG13 antibodies are essential tools for studying the molecular mechanisms of autophagy, a critical cellular degradation process. Autophagy-related protein 13 (ATG13) is a key scaffold component of the ULK1/2 kinase complex, which initiates autophagosome formation in response to nutrient deprivation or stress. This complex, comprising ULK1/2. ATG13. FIP200. and ATG101. integrates upstream signals from mTORC1 and AMPK to regulate autophagy induction. ATG13 undergoes phosphorylation at specific residues (e.g., S318. S554) depending on nutrient conditions, modulating its interaction with ULK1/2 and the complex’s activity. Antibodies targeting ATG13 are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect protein expression levels, post-translational modifications, and subcellular localization. Researchers also employ these antibodies to investigate autophagy dysregulation in diseases such as cancer, neurodegenerative disorders, and metabolic syndromes. However, variability in antibody specificity across species (human, mouse, rat) or isoforms (ATG13A/B) requires careful validation. Some studies highlight conflicting observations regarding ATG13’s phosphorylation-dependent roles, underscoring the importance of context-specific antibody applications. Overall, ATG13 antibodies remain indispensable for dissecting autophagy pathways and developing therapeutic strategies targeting this process.