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Rabbit Polyclonal EIF4A3 Antibody

  • 中文名: EIF4A3抗体
  • 别    名: RCPS; DDX48; MUK34; NUK34; NMP265; eIF4AIII
货号: IPDX09709
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/300 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

参考文献

以下是3-4条关于EIF4A3抗体的参考文献概览(文献名称和作者为模拟内容,供参考):

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1. **文献名称**:*EIF4A3 is a core component of the exon junction complex and regulates RNA splicing*

**作者**:Chan et al.

**摘要**:本研究利用EIF4A3特异性抗体进行免疫共沉淀实验,证实EIF4A3是外显子连接复合物(EJC)的核心蛋白,通过调控pre-mRNA剪接影响基因表达,并揭示了其DEAD-box结构域在结合RNA中的关键作用。

2. **文献名称**:*Overexpression of EIF4A3 in colorectal cancer correlates with poor prognosis*

**作者**:Wang et al.

**摘要**:通过免疫组化(使用EIF4A3多克隆抗体)分析结直肠癌组织,发现EIF4A3高表达与肿瘤转移和患者生存率下降显著相关,提示其可作为潜在预后标志物。

3. **文献名称**:*EIF4A3 inhibition suppresses SARS-CoV-2 replication by targeting viral RNA translation*

**作者**:Zhang et al.

**摘要**:研究通过siRNA和EIF4A3抗体敲低实验,证明EIF4A3通过与病毒RNA 5'UTR结合促进SARS-CoV-2蛋白翻译,为抗病毒治疗提供了新靶点。

4. **文献名称**:*EIF4A3 dysfunction triggers nonsense-mediated mRNA decay defects in neurodegenerative disorders*

**作者**:Smith et al.

**摘要**:利用EIF4A3抗体在小鼠脑组织中的免疫荧光实验,发现其异常定位导致无义介导的mRNA降解(NMD)通路受阻,可能与tau蛋白病理沉积相关。

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**注**:以上文献为示例性质,实际引用时建议通过PubMed或Google Scholar核对真实文献。检索时可使用关键词:**EIF4A3 antibody**, **EIF4A3 function**, **EIF4A3 disease**。

背景信息

The EIF4A3 antibody targets the eukaryotic initiation factor 4A-III (EIF4A3), a member of the DEAD-box RNA helicase family involved in RNA metabolism. EIF4A3 is a core component of the exon junction complex (EJC), which forms during pre-mRNA splicing and plays critical roles in mRNA surveillance, localization, and translation. Unlike its paralogs EIF4A1 and EIF4A2. EIF4A3 lacks direct involvement in canonical translation initiation but is essential for nonsense-mediated mRNA decay (NMD), a quality-control mechanism that degrades aberrant transcripts. Structurally, EIF4A3 binds ATP and RNA, utilizing helicase activity to remodel RNA-protein interactions within the EJC. Antibodies against EIF4A3 are widely used to study its function in RNA processing, EJC assembly, and NMD pathways. They are employed in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect protein expression, localization, and interactions. Dysregulation of EIF4A3 is linked to cancers and neurological disorders, making its antibody a valuable tool for investigating disease mechanisms. Research also explores EIF4A3's role in cellular stress responses and viral infections, highlighting its broader significance in post-transcriptional gene regulation.

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