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Rabbit Polyclonal ZFP36 Antibody

  • 中文名: ZFP36抗体
  • 别    名: TTP; G0S24; GOS24; TIS11; NUP475; zfp-36; RNF162A
货号: IPDX09220
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/30-1/150 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesTTP; G0S24; GOS24; TIS11; NUP475; zfp-36; RNF162A
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenFusion protein of human ZFP36
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于ZFP36抗体的3篇参考文献及其摘要概括:

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1. **文献名称**: *"Tristetraprolin (TTP) and its role in regulating mRNA stability in immune cells"*

**作者**: Carballo, E., Lai, W.S., Blackshear, P.J.

**摘要**: 该研究通过Western blot和免疫荧光技术,使用ZFP36/TTP特异性抗体,揭示其在巨噬细胞中通过结合促炎细胞因子mRNA(如TNF-α)并促进其降解,从而调控炎症反应的分子机制。

2. **文献名称**: *"Phosphorylation of Tristetraprolin by MAPKAP kinase 2 regulates its anti-inflammatory function"*

**作者**: Brook, M., Tchen, C.R., et al.

**摘要**: 研究利用抗ZFP36抗体进行免疫共沉淀实验,发现丝裂原活化蛋白激酶(MAPK)介导的ZFP36磷酸化修饰可抑制其与mRNA的结合能力,从而减弱其抗炎作用,为炎症性疾病治疗提供新靶点。

3. **文献名称**: *"Antibody-specific validation for ZFP36/TTP detection in human tissues"*

**作者**: Liang, J., Clark, A.R., et al.

**摘要**: 该文献系统评估了多种市售ZFP36抗体的特异性,通过siRNA敲低实验和质谱分析,确认部分抗体存在交叉反应性,强调了选择高特异性抗体在临床样本(如风湿性关节炎组织)检测中的重要性。

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以上文献均聚焦于ZFP36抗体的应用及功能研究,涵盖机制探索、翻译后修饰验证及抗体特异性评估等领域。

背景信息

ZFP36 (also known as Tristetraprolin or TTP) is an RNA-binding protein that plays a critical role in regulating inflammatory responses by destabilizing target mRNAs containing AU-rich elements (AREs) in their 3' untranslated regions. It is a member of the TIS11 family of zinc finger proteins and acts as a post-transcriptional suppressor of pro-inflammatory cytokines, such as TNF-α, IL-6. and GM-CSF, by promoting mRNA degradation. Dysregulation of ZFP36 has been linked to chronic inflammation, autoimmune diseases, and cancer.

Antibodies targeting ZFP36 are essential tools for studying its expression, localization, and functional interactions in various biological contexts. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate ZFP36's role in immune regulation, cellular stress responses, and disease mechanisms. Research utilizing ZFP36 antibodies has revealed its involvement in controlling mRNA stability during inflammation, highlighting its potential as a therapeutic target. Commercial ZFP36 antibodies are typically raised against specific epitopes of human or murine ZFP36. with validation in knockout models to ensure specificity. Their applications span immunology, oncology, and molecular biology, aiding in elucidating how post-transcriptional gene regulation impacts physiological and pathological processes.

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