| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ZBP-99; ZNP-99 |
| WB Predicted band size | 97 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ZNF281 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及ZNF281抗体的研究文献概览(基于近年研究整理)：
1. **"ZNF281 promotes colorectal cancer progression by regulating MYC via epigenetic repression of microRNAs"**
- **作者**: Zhang Y. et al.
- **摘要**: 研究揭示ZNF281通过表观遗传抑制miR-34a和miR-200家族激活MYC信号通路，促进结直肠癌增殖和转移。文中使用ZNF281抗体进行ChIP-seq和Western blot分析，验证其与靶基因启动子的直接结合。
2. **"Zinc finger protein 281 is a transcriptional activator of the SOX2 gene in breast cancer stem cells"**
- **作者**: Heidel F. et al.
- **摘要**: 发现ZNF281通过结合SOX2启动子维持乳腺癌干细胞干性，并促进化疗耐药。研究利用ZNF281特异性抗体进行免疫沉淀和免疫荧光染色，证实其在肿瘤组织中的高表达。
3. **"ZNF281 interacts with β-catenin/Tcf4 to enhance hepatocellular carcinoma progression"**
- **作者**: Wang L. et al.
- **摘要**: 报道ZNF281与β-catenin/Tcf4形成复合物，协同激活Wnt/β-catenin通路，驱动肝癌侵袭。研究通过免疫组化(ZNF281抗体)显示其高表达与患者不良预后相关。
4. **"The dual role of ZNF281 in DNA damage response and tumorigenesis"** (综述)
- **作者**: Chen X. et al.
- **摘要**: 综述ZNF281在调控DNA修复与肿瘤发生中的双重功能，强调其抗体在检测不同亚细胞定位(核/胞质)中的应用，及其作为潜在治疗靶点的价值。
**注**：实际文献需通过PubMed/Google Scholar检索确认，以上为基于领域研究的模拟概括。
ZNF281 (Zinc Finger Protein 281) is a transcription factor belonging to the Krüppel-associated box (KRAB) domain zinc finger protein family. It plays critical roles in regulating gene expression by binding to specific DNA sequences, primarily through its C-terminal zinc finger motifs. ZNF281 is involved in diverse cellular processes, including cell proliferation, differentiation, apoptosis, and the maintenance of stem cell pluripotency. Notably, it has been implicated in cancer biology, where it can act as an oncogene or tumor suppressor depending on the cellular context. For example, ZNF281 promotes tumor progression in colorectal cancer, breast cancer, and hepatocellular carcinoma by enhancing epithelial-mesenchymal transition (EMT), metastasis, or chemoresistance, while its downregulation is linked to poor prognosis in some malignancies.
Antibodies targeting ZNF281 are essential tools for studying its expression, localization, and molecular interactions. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Commercial ZNF281 antibodies are typically raised against specific epitopes within the N-terminal or zinc finger domains, available as monoclonal or polyclonal formats. Researchers rely on these antibodies to investigate ZNF281's role in transcriptional networks, its crosstalk with pathways like Wnt/β-catenin and p53. and its potential as a therapeutic target. Validation of antibody specificity (e.g., using knockout controls) is crucial due to possible cross-reactivity with other zinc finger proteins. Reliable ZNF281 antibodies advance studies in cancer, developmental biology, and regenerative medicine.
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