| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ZN473; ZFP100 |
| WB Predicted band size | 100 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ZNF473 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与ZNF473抗体相关的文献示例(注：文献信息为模拟概括，实际引用请以真实文献为准)：
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1. **文献名称**: *ZNF473 interacts with spliceosomal proteins and promotes RNA processing in human cells*
**作者**: Miyake, K. et al.
**摘要**: 研究报道ZNF473蛋白通过与剪接体核心组分(如PRPF8)相互作用，调控pre-mRNA剪接过程。文章使用ZNF473抗体进行免疫共沉淀实验，验证其在RNA加工中的功能，并发现其缺失导致剪接异常。
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2. **文献名称**: *Zinc finger protein 473 is a novel regulator of pluripotency in embryonic stem cells*
**作者**: Lee, S. & Zhu, H.
**摘要**: 本研究通过ZNF473抗体染色和ChIP-seq分析，发现ZNF473直接结合OCT4启动子区，维持胚胎干细胞多能性。敲低ZNF473导致多能性基因表达下调，提示其在干细胞自我更新中的关键作用。
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3. **文献名称**: *Antibody-based profiling of ZNF473 reveals overexpression in breast cancer and correlation with poor prognosis*
**作者**: Smith, J.R. & Johnson, A.L.
**摘要**: 利用ZNF473抗体对乳腺癌组织进行免疫组化分析，发现ZNF473在肿瘤组织中显著高表达，且与患者生存率负相关。机制研究表明其通过调控DNA修复通路促进肿瘤进展。
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4. **文献名称**: *Characterization of ZNF473 knockout mice using specific antibody validation*
**作者**: Nagase, T. et al.
**摘要**: 研究通过ZNF473抗体验证基因敲除小鼠模型，发现ZNF473缺失导致发育缺陷和基因组不稳定性，提示其在DNA损伤应答中的潜在功能。
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(注：以上为模拟文献，实际研究中请通过PubMed或Google Scholar以“ZNF473 antibody”为关键词检索真实文献。)
**Background of ZNF473 Antibody**
ZNF473 (Zinc Finger Protein 473) is a member of the zinc finger protein family, characterized by conserved C2H2-type zinc finger motifs that mediate DNA or RNA binding. This protein is implicated in transcriptional regulation, chromatin remodeling, and DNA repair processes. Studies suggest ZNF473 interacts with the spliceosome complex, potentially influencing pre-mRNA splicing, and associates with proteins involved in maintaining genomic stability, such as those in the Fanconi anemia pathway.
Antibodies targeting ZNF473 serve as critical tools for investigating its expression, localization, and molecular functions. These antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect endogenous ZNF473 in various cell types or tissues. Validated antibodies typically exhibit specificity for ZNF473 through knockout cell line controls, ensuring minimal cross-reactivity with related zinc finger proteins.
Research utilizing ZNF473 antibodies has highlighted its role in cellular stress responses and cancer biology. For instance, altered ZNF473 expression has been observed in malignancies, suggesting potential involvement in tumorigenesis or therapy resistance. Additionally, its interaction with DNA damage response pathways underscores its importance in maintaining genomic integrity. Commercial ZNF473 antibodies are often raised in rabbits or mice, with epitopes mapped to conserved regions of the protein. Proper validation remains essential for reliable experimental outcomes, particularly in studies exploring ZNF473's mechanistic contributions to disease.
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