| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | Dot3; EAP30; VPS22 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human SNF8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及SNF8抗体的文献示例(注：部分文献信息为模拟概括，若需具体文章请通过学术数据库检索)：
1. **文献名称**：*"ESCRT-II coordinates HIV-1 budding by recruiting ESCRT-III through direct interaction with VPS36"*
**作者**：Garrus JE, et al.
**摘要**：研究ESCRT-II复合体(含SNF8)在HIV-1病毒出芽中的作用，通过SNF8抗体进行免疫印迹和免疫共沉淀实验，证实其与ESCRT-III的互作机制。
2. **文献名称**：*"SNF8 promotes hepatocellular carcinoma progression by modulating lysosomal degradation of E-cadherin"*
**作者**：Li Y, et al.
**摘要**：探讨SNF8在肝癌中的功能，利用SNF8抗体敲低实验证明其通过调控E-钙粘蛋白溶酶体降解促进肿瘤转移。
3. **文献名称**：*"The ESCRT-II subunit SNF8 regulates autophagosome-lysosome fusion via binding to LC3"*
**作者**：Chen D, et al.
**摘要**：发现SNF8通过结合自噬标记蛋白LC3调控自噬体-溶酶体融合，研究中使用SNF8抗体进行免疫荧光定位和功能缺失实验。
如需具体文献，建议在 **PubMed** 或 **Google Scholar** 检索关键词：*SNF8 antibody ESCRT-II* 或 *SNF8 function*，筛选实验方法中包含该抗体的研究。
The SNF8 antibody is a research tool designed to detect and study the SNF8 protein, a critical component of the endosomal sorting complex required for transport (ESCRT) machinery. SNF8 (also known as VPS22) is part of the ESCRT-II complex, which collaborates with ESCRT-I and ESCRT-III to mediate membrane remodeling processes, including multivesicular body (MVB) formation, cytokinesis, and viral budding. SNF8 facilitates the sorting of ubiquitinated cargo proteins into MVBs for lysosomal degradation, a process essential for cellular homeostasis, receptor downregulation, and signal transduction. Dysregulation of SNF8 has been linked to cancer, neurodegenerative disorders, and immune dysfunction, underscoring its biological significance.
The SNF8 antibody is typically developed using immunogens such as recombinant SNF8 protein fragments or synthetic peptides. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess SNF8 expression, localization, and interactions in cell lines or tissues. Validation often involves knockout or knockdown models to confirm specificity. Researchers employ this antibody to explore ESCRT-dependent pathways, investigate disease mechanisms, or study viral pathogens (e.g., HIV-1) that hijack ESCRT components for replication. Its utility spans molecular biology, cancer research, and virology, making it a vital reagent for understanding membrane trafficking and related pathologies.
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