| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PI; DFN7; GST3; FAEES3 |
| Entrez GeneID | 2950 |
| clone | 3F2C2 |
| WB Predicted band size | 23kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GSTP1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RPL8抗体的3篇代表性文献(示例为虚构内容，仅供参考格式)：
1. **文献名称**: "Characterization of a Novel Monoclonal Antibody Against Human RPL8 for Ribosomal Profiling"
**作者**: Smith A, et al.
**摘要**: 该研究开发了一种针对人源RPL8蛋白的单克隆抗体，验证了其在免疫印迹(WB)和免疫荧光(IF)中的特异性，并用于探究核糖体组装异常与癌症的关系。
2. **文献名称**: "RPL8 Expression and Its Interaction with Viral RNA in Hepatitis C Infection"
**作者**: Lee J, et al.
**摘要**: 通过RPL8抗体检测发现，RPL8在丙肝病毒感染的肝细胞中表达上调，且与病毒RNA结合可能调控病毒复制，提示其作为治疗靶点的潜力。
3. **文献名称**: "Role of RPL8 in Colorectal Cancer Progression: Insights from Antibody-Based Tissue Microarray Analysis"
**作者**: Chen X, et al.
**摘要**: 利用RPL8抗体对结直肠癌组织进行染色，发现RPL8高表达与患者预后不良相关，可能通过激活mTOR通路促进肿瘤生长。
(注：以上文献为示例，实际引用需检索PubMed或Google Scholar获取真实文献。)
The RPL8 antibody is a tool used to detect ribosomal protein L8 (RPL8), a component of the 60S ribosomal subunit involved in protein synthesis. RPL8. encoded by the RPL8 gene in humans, plays a structural role in ribosome assembly and facilitates mRNA binding during translation. Antibodies targeting RPL8 are widely utilized in research to study ribosomal biogenesis, cellular stress responses, and diseases linked to ribosomal dysfunction, such as cancer and genetic disorders like Diamond-Blackfan anemia.
In cancer biology, RPL8 expression is often dysregulated, and its detection helps assess tumor progression or therapy resistance. The antibody is employed in techniques like Western blotting, immunofluorescence, and immunohistochemistry to localize and quantify RPL8 in tissues or cell lines. It also aids in investigating post-translational modifications of RPL8 under stress conditions, such as nutrient deprivation or oxidative stress.
Commercially available RPL8 antibodies are typically raised in rabbits or mice using synthetic peptides or recombinant proteins as immunogens. Validation includes testing for specificity via knockout cells or siRNA-mediated silencing. Researchers must optimize conditions (e.g., dilution, fixation) to minimize cross-reactivity with homologous ribosomal proteins. Overall, the RPL8 antibody serves as a critical reagent for exploring ribosome-related mechanisms in both basic and translational research contexts.
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