| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/150-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | TP1; TLP1; p240; TROVE1; VAULT2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human TEP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TEP1抗体的3篇代表性文献(信息基于公开研究整合，部分细节可能需核实)：
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1. **文献名称**：*TEP1 protein: A component of the telomerase complex essential for enzyme activity*
**作者**：Harrington, L., & Robinson, M. O. (1997)
**摘要**：该研究首次鉴定TEP1(端粒酶相关蛋白1)为端粒酶复合体的核心成分，利用特异性抗体证明其与端粒酶催化亚基(TERT)结合，并证实TEP1缺失会导致端粒酶活性显著下降。
2. **文献名称**：*Functional analysis of TEP1 in telomerase assembly and RNA binding*
**作者**：Cohen, S. B., & Blackburn, E. H. (1998)
**摘要**：通过TEP1抗体的免疫沉淀实验，揭示了TEP1在端粒酶RNA(TERC)结合和复合体稳定性中的关键作用，证明其结构域对端粒酶功能不可或缺。
3. **文献名称**：*TEP1 antibody-based detection of telomerase complexes in human cancer cells*
**作者**：Vulliamy, T., et al. (2001)
**摘要**：研究开发了高特异性TEP1单克隆抗体，用于检测多种癌症细胞系中端粒酶复合体的表达水平，发现TEP1表达与端粒酶活性及肿瘤恶性程度正相关。
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**注意**：以上文献信息为模拟示例，实际引用需根据具体论文数据库(如PubMed)检索并核对原文。建议通过关键词“TEP1 antibody”、“telomerase-associated protein 1”等进一步筛选最新研究。
TEP1 (Telomerase-associated protein 1), also known as TERC-binding protein, is a critical component of the telomerase complex, which plays a pivotal role in maintaining telomere integrity and cellular immortality. Telomerase counteracts telomere shortening during DNA replication by adding repetitive nucleotide sequences to chromosome ends. The TEP1 protein interacts with the telomerase RNA component (TERC) and the catalytic subunit TERT (telomerase reverse transcriptase), stabilizing the complex and facilitating its nuclear localization and enzymatic activity. While TEP1 is not directly involved in the catalytic function of telomerase, it is essential for telomerase assembly and RNA binding.
Antibodies targeting TEP1 are widely used in research to investigate telomerase biology, particularly in studies related to aging, cancer, and stem cell regulation. These antibodies enable the detection and quantification of TEP1 expression in various tissues and cell types through techniques like Western blotting, immunoprecipitation, and immunofluorescence. Dysregulation of TEP1 has been implicated in telomerase-deficient disorders and oncogenesis, making it a biomarker of interest in cancer diagnostics and therapeutic development. Notably, TEP1 homologs are evolutionarily conserved across species, underscoring its fundamental role in telomere maintenance. Research using TEP1 antibodies continues to elucidate its molecular interactions and potential clinical applications in age-related diseases and malignancies.
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