| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | GS2; RAM; RAB27; HsT18676 |
| WB Predicted band size | 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RAB27A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RAB27A抗体的3篇代表性文献摘要(文献名称与内容为模拟概括，实际文献需通过数据库验证)：
1. **文献名称**：*RAB27A regulates exocytosis of lysosome-related organelles in melanocytes*
**作者**：Hume AN, et al.
**摘要**：研究利用RAB27A抗体验证其在黑色素细胞中的定位及功能，揭示RAB27A通过调控溶酶体相关细胞器(如黑色素体)的胞吐作用影响皮肤色素运输，突变可导致Griscelli综合征。
2. **文献名称**：*Mutations in RAB27A cause Griscelli syndrome associated with haemophagocytic syndrome*
**作者**：Menasche G, et al.
**摘要**：通过RAB27A抗体检测患者细胞中蛋白表达缺失，首次证实RAB27A基因突变与Griscelli综合征(免疫缺陷与色素异常)的直接关联，阐明其在细胞毒性T细胞颗粒分泌中的关键作用。
3. **文献名称**：*Slp4-a/granuphilin regulates insulin exocytosis through interaction with RAB27A in pancreatic β cells*
**作者**：Fukuda M.
**摘要**：研究利用RAB27A抗体进行免疫共沉淀实验，发现RAB27A与其效应蛋白Slp4-a的相互作用调控胰岛素分泌囊泡的胞吐机制，为糖尿病相关分泌障碍提供分子机制解释。
4. **文献名称**：*RAB27A controls melanosome distribution by interacting with myosin Va via melanophilin*
**作者**：Bahadoran P, et al.
**摘要**：通过抗体标记和共聚焦成像，证明RAB27A通过连接黑色素体与肌球蛋白Va复合物，驱动其在细胞内的运输，缺失会导致黑色素颗粒异常聚集。
(注：以上内容基于领域内经典研究主题的概括，实际文献信息请通过PubMed或Google Scholar检索确认。)
The RAB27A antibody is a crucial tool in studying the RAB27A protein, a member of the RAS-associated binding (RAB) GTPase family involved in regulating intracellular vesicle trafficking, secretion, and membrane fusion. RAB27A plays a key role in lysosome-related organelles, melanosome transport in melanocytes, and granule exocytosis in immune cells (e.g., cytotoxic T cells and platelets). Dysregulation of RAB27A is linked to genetic disorders like Griscelli syndrome type 2 (characterized by hypopigmentation and immunodeficiency) and cancers (e.g., melanoma, leukemia), where it influences metastasis, immune evasion, and extracellular vesicle release.
RAB27A antibodies are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). They help elucidate molecular mechanisms in cellular trafficking pathways, immune responses, and disease pathogenesis. Commercial RAB27A antibodies are typically raised in rabbits or mice, targeting specific epitopes (e.g., N-terminal or C-terminal regions). Validation includes testing in knockout cell lines or tissues to confirm specificity. Researchers also employ these antibodies to explore therapeutic targets, such as blocking RAB27A-mediated exosome release in tumor microenvironments. Their applications span cell biology, immunology, oncology, and dermatology, making them indispensable for understanding vesicular transport dynamics and related pathologies.
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