| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | NPAP60; NPAP60L |
| WB Predicted band size | 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human NUP50 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NUP50抗体的模拟参考文献示例(实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*NUP50 overexpression promotes breast cancer progression by modulating nuclear transport*
**作者**：Smith A, et al.
**摘要**：研究利用NUP50抗体进行免疫组化分析，发现乳腺癌组织中NUP50蛋白显著高表达，并通过调控核质运输促进肿瘤细胞增殖和侵袭。
2. **文献名称**：*Role of NUP50 in cell cycle regulation and mitotic progression*
**作者**：Lee J, et al.
**摘要**：通过NUP50抗体的免疫荧光染色和Western blot，揭示了NUP50在细胞周期中的动态定位变化，及其与有丝分裂关键蛋白的相互作用机制。
3. **文献名称**：*NUP50 interacts with HIV-1 capsid to facilitate viral nuclear entry*
**作者**：Zhang Y, et al.
**摘要**：使用NUP50抗体的免疫共沉淀实验，证实了NUP50与HIV-1衣壳蛋白的相互作用，揭示了其在病毒核内运输中的关键作用。
4. **文献名称**：*Developmental expression of NUP50 in mouse embryonic tissues*
**作者**：Tanaka K, et al.
**摘要**：通过NUP50抗体的免疫组织化学分析，系统描述了NUP50在小鼠胚胎不同发育阶段的组织特异性表达模式，提示其在器官形成中的潜在功能。
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如需实际文献，建议通过PubMed或Google Scholar检索关键词“NUP50 antibody”或“NUP50 nucleoporin”，筛选实验方法部分明确使用该抗体的研究。
NUP50 (Nucleoporin 50), also known as NPHS5 or nuclear pore complex protein Nup50. is a key component of the nuclear pore complex (NPC), a large protein assembly embedded in the nuclear envelope that regulates nucleocytoplasmic transport. It belongs to the nucleoporin family, which facilitates selective trafficking of macromolecules between the nucleus and cytoplasm. Structurally, NUP50 contains an N-terminal α-helical domain, a central FG (phenylalanine-glycine) repeat region, and a C-terminal Ran-binding domain. These features enable interactions with transport receptors (e.g., importins/exportins) and the small GTPase Ran, critical for directing cargo dissociation or binding during transport cycles.
Functionally, NUP50 plays roles in mRNA export, post-mitotic nuclear pore assembly, and chromatin organization. It also participates in nuclear import by promoting the release of importin α from cargo proteins, ensuring efficient recycling of transport factors. Dysregulation of NUP50 has been linked to human diseases, including premature ovarian insufficiency (POI) and glomerulopathies, due to mutations affecting its interactions with other nucleoporins or transport machinery.
NUP50 antibodies are widely used tools in research to study NPC architecture, nuclear transport mechanisms, and disease-related alterations. These antibodies, often targeting specific domains (e.g., FG repeats or C-terminal regions), enable applications such as immunofluorescence, Western blotting, and immunoprecipitation. Commercial clones (e.g., H-9 by Proteintech or E-6 by Santa Cruz) vary in host species (mouse, rabbit) and validation standards, requiring careful selection based on experimental needs. Recent studies also explore NUP50's role in cancer, neurodegeneration, and reproductive disorders, highlighting its broader biomedical relevance.
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