Rabbit Polyclonal PABPC1 Antibody

Poly(A)-binding protein cytoplasmic 1 (PABPC1) is a highly conserved RNA-binding protein critical for post-transcriptional gene regulation. It binds the poly(A) tails of eukaryotic mRNAs, stabilizing transcripts and promoting translation by interacting with translation initiation factors (e.g., eIF4G) and circularizing mRNAs via PABPC1-eIF4E interactions. PABPC1 also participates in mRNA turnover, microRNA-mediated silencing, and stress granule formation. Unlike nuclear PABPN1. PABPC1 localizes predominantly to the cytoplasm. Antibodies targeting PABPC1 are widely used to study its roles in mRNA metabolism, cellular stress responses, viral infections (e.g., enteroviruses that cleave PABPC1), and diseases such as cancer and neurodegeneration. These antibodies (often rabbit or mouse monoclonal/polyclonal) enable applications like Western blotting, immunofluorescence, immunoprecipitation, and RNA-protein interaction assays. Commercial PABPC1 antibodies are typically validated for specificity using knockdown controls or recombinant protein blocking. Researchers also employ them to investigate PABPC1 dysregulation in conditions like oculopharyngeal muscular dystrophy (linked to PABPN1 mutations) or inflammation, where PABPC1 modulates immune-related mRNA stability. Proper validation remains essential due to cross-reactivity risks with other PABP family members or isoforms.