| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MME; NEP; SFE; CALLA |
| Entrez GeneID | 4311 |
| clone | 7D4B1 |
| WB Predicted band size | 85.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD10 (AA: 52-246) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于LDHD抗体的3篇模拟参考文献(基于已有知识推测，实际文献可能需要通过数据库验证)：
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1. **文献名称**：*"Lactate Dehydrogenase D (LDHD) as a Novel Prognostic Biomarker in Hepatocellular Carcinoma: Insights from Immunohistochemical Analysis"*
**作者**：Zhang Y, Wang L, et al. (2021)
**摘要**：本研究通过LDHD特异性抗体检测肝癌组织中LDHD蛋白的表达水平，发现LDHD高表达与患者预后不良显著相关，提示其可能作为肝癌的新型生物标志物及潜在治疗靶点。
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2. **文献名称**：*"Structural Characterization and Antibody Development for Human LDHD: Implications for Metabolic Disorders"*
**作者**：Smith J, Brown K, et al. (2020)
**摘要**：文章解析了LDHD蛋白的晶体结构，并基于其表位设计多克隆抗体。实验证实该抗体在Western blot和免疫组化中具有高特异性，为研究LDHD在代谢性疾病中的功能提供了工具。
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3. **文献名称**：*"LDHD Expression in Colorectal Cancer: Correlation with Glycolytic Activity and Clinical Outcomes"*
**作者**：Chen H, Li X, et al. (2019)
**摘要**：利用LDHD抗体对结直肠癌样本进行染色，发现LDHD表达水平与肿瘤糖酵解活性升高相关，且高表达患者对化疗反应较差，表明其在肿瘤代谢重编程中的作用。
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**备注**：以上文献信息为示例，实际引用请通过PubMed、Web of Science等数据库检索关键词(如LDHD antibody、lactate dehydrogenase D)获取最新研究。
LDHD (L-lactate dehydrogenase D) antibodies are immunological tools used to detect and study the LDHD protein, a recently characterized member of the lactate dehydrogenase (LDH) family. Unlike the well-known LDHA and LDHB isoforms involved in anaerobic glycolysis, LDHD is primarily expressed in mitochondria and plays a distinct role in lactate metabolism. It catalyzes the oxidation of L-lactate to pyruvate, linking cytoplasmic glycolysis with mitochondrial oxidative phosphorylation. LDHD’s discovery expanded the understanding of lactate shuttle mechanisms and metabolic flexibility in cells.
Research on LDHD has gained momentum due to its potential implications in metabolic disorders, cancer, and age-related diseases. Dysregulation of LDHD is associated with altered lactate homeostasis, which may influence tumor progression, neurodegeneration, and mitochondrial dysfunction. LDHD antibodies are critical for investigating its expression patterns, subcellular localization, and interactions in these contexts. They are commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence.
Commercial LDHD antibodies are typically raised in rabbits or mice against specific peptide epitopes. Validation includes testing in knockout models or siRNA-treated cells to confirm specificity, as cross-reactivity with other LDH isoforms is a common challenge. Ongoing studies aim to clarify LDHD’s physiological roles and therapeutic potential, making these antibodies essential for advancing metabolic and biomedical research.
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