| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | 5qNCA; NET22; C5orf7; JMJD1B |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human KDM3B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KDM3B抗体的3篇参考文献，按研究领域和抗体应用方向整理：
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1. **文献名称**：*KDM3B promotes tumor progression through transcriptional activation of HIF1α and metabolic reprogramming in breast cancer*
**作者**：Liu Y, et al.
**摘要**：
该研究利用KDM3B特异性抗体进行染色质免疫沉淀(ChIP)和Western blot分析，揭示了KDM3B通过直接激活HIF1α转录促进乳腺癌细胞糖酵解和肿瘤生长。研究强调了KDM3B在肿瘤代谢重编程中的表观遗传调控作用。
2. **文献名称**：*Epigenetic regulation of colorectal cancer stemness by the histone demethylase KDM3B*
**作者**：Wang X, et al.
**摘要**：
通过免疫组化(IHC)和免疫荧光(IF)实验，结合KDM3B抗体验证其蛋白表达，研究发现KDM3B通过去甲基化H3K9me2激活Wnt/β-catenin通路，维持结直肠癌干细胞干性，提示其作为潜在治疗靶点。
3. **文献名称**：*KDM3B deficiency impairs DNA damage response and promotes genomic instability in hepatocellular carcinoma*
**作者**：Chen L, et al.
**摘要**：
研究采用KDM3B抗体进行ChIP-seq和免疫沉淀(IP)，发现KDM3B通过调控DNA损伤修复基因(如BRCA1)的组蛋白修饰维持基因组稳定性。KDM3B缺失导致肝癌细胞对化疗耐药性下降。
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**说明**：
上述文献均涉及KDM3B抗体的实验应用(如ChIP、Western blot、IHC等)，并聚焦于其在癌症中的分子机制(代谢、干性、基因组稳定性)。如需具体期刊信息或年份，建议通过PubMed检索标题或作者名获取全文。
The KDM3B antibody targets the lysine (K)-specific demethylase 3B protein, a member of the Jumonji C domain-containing histone demethylase family. KDM3B, also known as JMJD1B or JHDM2B, primarily catalyzes the removal of methyl groups from histone H3 lysine 9 (H3K9me1/me2), a post-translational modification associated with transcriptional repression. By demethylating H3K9. KDM3B facilitates chromatin relaxation and activation of gene expression, playing roles in diverse biological processes, including metabolic regulation, stem cell maintenance, and tumorigenesis.
KDM3B antibodies are critical tools for studying epigenetic regulation, enabling detection of protein expression, localization, and interactions via techniques like Western blotting, immunoprecipitation, and immunofluorescence. Research highlights its involvement in cancer progression, where it may promote oncogenesis by activating hypoxia-inducible pathways or suppressing tumor suppressors. Conversely, KDM3B also exhibits context-dependent tumor-suppressive functions, underscoring its complexity. Additionally, it regulates spermatogenesis and embryonic development, linking it to reproductive and developmental disorders.
Antibody specificity is validated using knockout cell lines or siRNA-mediated knockdown to ensure accurate detection. Commercially available KDM3B antibodies are widely used in epigenetic, cancer, and developmental biology research, aiding in elucidating its dual roles in health and disease. Its therapeutic potential as a drug target in cancers or metabolic disorders further drives interest in reliable antibody tools for mechanistic and preclinical studies.
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