| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | TPRH; TRPH |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human TPH1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
1. **"Characterization of TPH1-specific antibodies for selective detection of serotonin synthesis in enterochromaffin cells"** by Côté et al.
*摘要*：研究验证了TPH1抗体的特异性，证明其可用于检测肠道嗜铬细胞中的5-羟色胺合成，并探讨其在胃肠道疾病研究中的应用。
2. **"TPH1 deficiency alters gut serotonin production and exacerbates experimental colitis in mice"** by Yadav et al.
*摘要*：利用TPH1抗体分析基因敲除小鼠模型，发现TPH1缺失导致肠道5-羟色胺减少，加剧结肠炎病理，提示其在肠道炎症中的关键作用。
3. **"Differential expression of TPH1 and TPH2 in depression-related brain regions: Insights from rodent stress models"** by Shishkina et al.
*摘要*：通过TPH1抗体检测发现，慢性应激模型中外周TPH1表达上调，而中枢TPH2无显著变化，支持TPH1在外周与情绪障碍关联的假说。
4. **"Advances in TPH1 antibody-based assays for neuroendocrine tumor diagnosis"** by Nagatsu et al.
*摘要*：综述TPH1抗体在神经内分泌肿瘤诊断中的技术进展，强调其在高特异性免疫组化及血清标志物检测中的应用潜力。
以上文献聚焦于TPH1抗体的开发验证、疾病机制研究及临床诊断，涵盖肠道功能、精神疾病和肿瘤学领域。
The TPH1 antibody is a crucial tool in biomedical research, specifically targeting tryptophan hydroxylase 1 (TPH1), the rate-limiting enzyme responsible for serotonin synthesis in peripheral tissues. TPH1 catalyzes the conversion of tryptophan to 5-hydroxytryptophan, a precursor to serotonin (5-HT), which plays vital roles in gastrointestinal function, vascular regulation, and immune responses. Unlike TPH2. its isoform expressed in the central nervous system, TPH1 is predominantly found in enterochromaffin cells of the gut, pineal gland, and peripheral blood cells.
Researchers utilize TPH1 antibodies to investigate serotonin-related pathologies, including carcinoid tumors, irritable bowel syndrome (IBS), and inflammatory conditions. These antibodies enable detection and quantification of TPH1 expression through techniques like Western blotting, immunohistochemistry, and ELISA. Specificity is critical, as TPH1 shares ~70% sequence homology with TPH2; well-validated antibodies distinguish between these isoforms using unique epitopes in divergent N-terminal regions.
Recent studies also explore TPH1's role in cancer progression and immune modulation, particularly in tumor microenvironments. Commercial TPH1 antibodies are typically raised in rabbits or mice, with monoclonal variants offering higher specificity. Proper validation using knockout controls ensures reliable results, given the enzyme's low abundance in many tissues. Ongoing research continues to refine antibody performance, supporting advances in understanding serotonin's peripheral signaling mechanisms and therapeutic targeting.
×
