| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ARTD2; ADPRT2; PARP-2; ADPRTL2; ADPRTL3; pADPRT-2 |
| WB Predicted band size | 66 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human PARP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与PARP2抗体相关的文献摘要(虚拟模拟数据，仅供示例参考)：
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1. **文献名称**：*Development of a High-Specificity PARP2 Antibody for Detecting DNA Damage Response in Human Cells*
**作者**：Smith A et al.
**摘要**：本研究开发了一种靶向PARP2 C端结构域的单克隆抗体，验证了其在免疫印迹和免疫荧光中的特异性，证明其能有效区分PARP2与PARP1.适用于DNA损伤修复过程的动态追踪。
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2. **文献名称**：*PARP2 Inhibition Sensitizes Ovarian Cancer to Chemotherapy: Insights from Antibody-Based Functional Studies*
**作者**：Chen L et al.
**摘要**：通过PARP2特异性抗体阻断实验，发现抑制PARP2可增强卵巢癌细胞对铂类药物的敏感性，揭示了PARP2在化疗耐药中的独立于PARP1的调控机制。
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3. **文献名称**：*Comparative Analysis of PARP2 Antibody Performance in Neurodegenerative Disease Models*
**作者**：Gomez-Ramos P et al.
**摘要**：系统比较了市售PARP2抗体的特异性与适用性，发现克隆号X45的抗体在阿尔茨海默病小鼠模型中能精准检测到PARP2的异常活化，提示其作为神经炎症生物标志物的潜力。
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**注**：以上为模拟数据，实际文献需通过PubMed/Google Scholar检索关键词“PARP2 antibody”、“PARP2 validation”等获取。
Poly(ADP-ribose) polymerase 2 (PARP2) is a member of the PARP enzyme family, which plays critical roles in DNA repair, genome stability, and cellular stress responses. PARP2 shares structural and functional similarities with PARP1. including a conserved catalytic domain responsible for poly(ADP-ribosyl)ation (PARylation), a post-translational modification involved in DNA damage signaling. However, PARP2 has distinct N-terminal domains that influence its substrate specificity and interaction partners. It participates in base excision repair (BER), chromatin remodeling, and transcriptional regulation, with emerging roles in inflammation, metabolism, and cell death pathways.
PARP2-specific antibodies are essential tools for studying its expression, localization, and function in physiological and pathological contexts. These antibodies enable detection of PARP2 via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Given the therapeutic relevance of PARP inhibitors in cancer (e.g., targeting PARP1/2 for synthetic lethality in BRCA-deficient tumors), PARP2 antibodies are also used to evaluate drug-target engagement, PARylation dynamics, and compensatory mechanisms in resistant cancers. Recent studies highlight PARP2's involvement in neurodegenerative diseases and metabolic syndromes, expanding its research scope.
Validated PARP2 antibodies should distinguish PARP2 from PARP1 and other family members, requiring careful epitope selection. Both monoclonal and polyclonal variants are available, often targeting unique regions like the N-terminal regulatory domain. Controls (e.g., PARP2-knockout cells) are critical to ensure specificity, as cross-reactivity remains a common challenge. Overall, PARP2 antibodies are vital for unraveling its diverse biological roles and optimizing PARP-targeted therapies.
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