| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HOX4; HOX4C; Hox-4.3; Hox-5.2 |
| WB Predicted band size | 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human HOXD9 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及HOXD9抗体的研究文献摘要示例(注：文献信息为模拟概括，具体引用请核实原始论文)：
1. **《HOXD9 promotes glioma cell invasion via NF-κB signaling pathway》**
- 作者：Li Y, et al.
- 摘要：研究通过Western blot和免疫组化使用HOXD9抗体，发现HOXD9在胶质瘤中高表达，并通过激活NF-κB通路促进肿瘤侵袭。
2. **《HOXD9 acts as an oncogene in breast cancer by inducing EMT》**
- 作者：Wang X, et al.
- 摘要：利用HOXD9抗体进行染色质免疫沉淀(ChIP)，揭示HOXD9通过调控Snail表达诱导上皮间质转化(EMT)，从而驱动乳腺癌转移。
3. **《Prognostic value of HOXD9 in colorectal cancer and its role in angiogenesis》**
- 作者：Chen H, et al.
- 摘要：通过免疫组化分析HOXD9抗体标记的肿瘤样本，发现HOXD9高表达与结直肠癌患者预后不良相关，且促进血管生成相关基因表达。
4. **《HOXD9 antibody-based screening identifies its regulatory role in myeloid differentiation》**
- 作者：Zhang R, et al.
- 摘要：研究利用HOXD9抗体进行流式细胞术和荧光染色，证实HOXD9通过抑制特定转录因子影响髓系细胞分化过程。
(提示：实际文献需通过PubMed/Google Scholar检索关键词“HOXD9 antibody”或“HOXD9 function”获取，并核对实验方法部分是否明确使用该抗体。)
The HOXD9 antibody is a research tool designed to detect and study the HOXD9 protein, a member of the homeobox (HOX) gene family. HOX genes encode transcription factors critical for embryonic development, particularly in establishing body patterning and cellular differentiation. HOXD9. located on chromosome 2q31. regulates target genes by binding to specific DNA sequences, influencing processes like limb formation, skeletal development, and organogenesis. Dysregulation of HOXD9 has been implicated in cancer, where it may act as an oncogene or tumor suppressor depending on context. For example, elevated HOXD9 expression is linked to metastasis in breast and colorectal cancers, while its downregulation is observed in gliomas.
HOXD9 antibodies are typically monoclonal or polyclonal reagents produced in hosts like rabbits or mice. They enable the visualization and quantification of HOXD9 protein levels in techniques such as Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies are essential for exploring HOXD9's role in developmental biology, disease mechanisms, and potential therapeutic targeting. Researchers must validate antibody specificity using knockout controls or peptide-blocking assays, as cross-reactivity with other HOX family members is a common concern. Recent studies also highlight HOXD9's involvement in cellular senescence and stem cell regulation, expanding its relevance beyond developmental contexts. Proper antibody selection and optimization are crucial for reliable experimental outcomes in both basic and translational research.
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