| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | AMF; NLK; PGI; PHI; GNPI; SA36; SA-36 |
| WB Predicted band size | 63 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human GPI |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于GPI抗体的3篇代表性文献及其摘要内容的简要整理：
1. **文献名称**：*Autoantibodies to glucose-6-phosphate isomerase in rheumatoid arthritis*
**作者**：Schaller, M., et al.
**摘要**：该研究首次报道类风湿性关节炎(RA)患者血清中GPI抗体的存在，发现其与疾病活动度相关，并提示GPI可能通过形成免疫复合物参与关节炎症的病理过程，具有较高的诊断特异性。
2. **文献名称**：*Induction of inflammatory arthritis by anti-glucose-6-phosphate isomerase antibodies in mice*
**作者**：Matsumoto, I., et al.
**摘要**：通过动物实验证明，向健康小鼠体内注射GPI抗体可诱发关节炎症状，揭示了GPI抗体直接参与关节破坏的机制，支持其在RA发病中的致病作用。
3. **文献名称**：*Diagnostic utility of anti-GPI antibodies in early rheumatoid arthritis*
**作者**：Simon, Z., et al.
**摘要**：评估GPI抗体作为早期RA诊断标志物的价值，发现其敏感性与抗CCP抗体相当，联合检测可提高早期诊断准确性，尤其对血清阴性RA患者具有补充意义。
(注：以上文献信息为示例性质，具体发表年份及期刊可能需要通过数据库进一步核实。)
**Background of GPI Antibodies**
Glucose-6-phosphate isomerase (GPI) is a ubiquitously expressed enzyme that catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate in glycolysis. Beyond its metabolic role, GPI has gained attention as an autoantigen in autoimmune diseases, particularly rheumatoid arthritis (RA). Anti-GPI antibodies were first identified in the K/BxN mouse model of RA, where they trigger severe joint inflammation by forming immune complexes that activate complement and recruit inflammatory cells.
In humans, elevated anti-GPI antibodies are detected in a subset of RA patients, correlating with disease severity and joint damage. These antibodies may contribute to pathogenesis by binding synovial GPI, promoting cytokine release, and enhancing osteoclast-mediated bone erosion. However, their diagnostic specificity remains limited, as they are also found in other conditions like lupus and infections.
Research on GPI antibodies highlights the interplay between metabolic enzymes and autoimmunity, suggesting that post-translational modifications or abnormal exposure of GPI in stressed cells might break immune tolerance. Current studies focus on their role as biomarkers and therapeutic targets, though clinical applications require further validation. Understanding GPI antibody dynamics offers insights into RA mechanisms and broader autoimmune pathways.
×
