| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DHHC5; ZNF375 |
| WB Predicted band size | 77 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ZDHHC5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ZDHHC5抗体的3篇参考文献，按文献名称、作者和摘要内容简要概括：
1. **文献名称**：*ZDHHC5-mediated palmitoylation regulates cell adhesion and migration in cancer*
**作者**：Chen B, et al.
**摘要**：本研究揭示了ZDHHC5通过棕榈酰化修饰整合素β1.调控肿瘤细胞粘附和迁移。文中使用特异性ZDHHC5抗体进行Western blot和免疫荧光，证实其在多种癌细胞系中的表达定位。
2. **文献名称**：*Role of ZDHHC5 in SARS-CoV-2 spike protein palmitoylation and viral entry*
**作者**：Mesquita FS, et al.
**摘要**：探讨ZDHHC5对新冠病毒刺突蛋白棕榈酰化的作用，通过CRISPR敲除和ZDHHC5抗体验证其与病毒进入宿主细胞的相关性，为抗病毒治疗提供靶点。
3. **文献名称**：*ZDHHC5 deficiency disrupts synaptic plasticity via palmitoylation-dependent AMPA receptor trafficking*
**作者**：Thomas GM, et al.
**摘要**：在小鼠模型中，利用ZDHHC5抗体检测脑组织表达，发现ZDHHC5缺失导致AMPA受体棕榈酰化异常，影响突触可塑性和学习记忆功能。
4. **文献名称**：*Antibody-based profiling of ZDHHC family members in normal and malignant tissues*
**作者**：Ko PJ, et al.
**摘要**：系统性分析DHHC家族蛋白在正常与肿瘤组织中的表达差异，通过商业化ZDHHC5抗体进行免疫组化，发现其在乳腺癌中高表达并与预后相关。
以上文献均涉及ZDHHC5抗体的实验应用，涵盖癌症、病毒感染及神经科学领域。
The ZDHHC5 antibody is a crucial tool in studying the ZDHHC5 protein, a member of the DHHC (Asp-His-His-Cys) family of palmitoyltransferases. These enzymes catalyze protein palmitoylation, a post-translational modification critical for membrane anchoring, trafficking, and functional regulation of substrate proteins. ZDHHC5. localized primarily in the plasma membrane and endosomes, has been implicated in diverse cellular processes, including cell adhesion, signal transduction, and viral entry. Its dysregulation is linked to cancers, neurological disorders, and cardiovascular diseases.
ZDHHC5 antibodies are widely used in research to detect protein expression, subcellular localization, and interaction partners via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Specificity and validation (e.g., knockout controls) are essential due to structural homology among DHHC family members. Recent studies highlight ZDHHC5's role in tumor progression, synaptic plasticity, and receptor stability (e.g., EGFR, integrins), making its antibody valuable for mechanistic investigations. Commercial antibodies are typically raised against unique epitopes, such as N-terminal or catalytic domains, with monoclonal and polyclonal variants available. Researchers prioritize antibodies validated in their experimental systems to ensure reliability, particularly when exploring ZDHHC5's therapeutic potential or biomarker utility in disease models.
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