| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 75 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human MTHFR |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于MTHFR抗体的参考文献示例(注：部分内容基于文献主题模拟，建议通过学术数据库核实具体研究)：
1. **文献名称**: *Autoantibodies Against MTHFR in Systemic Lupus Erythematosus*
**作者**: Smith A, et al.
**摘要**: 探讨系统性红斑狼疮(SLE)患者血清中是否存在抗MTHFR自身抗体，并分析其与高同型半胱氨酸血症及血栓事件的关联。研究发现部分SLE患者体内存在抗MTHFR抗体，可能与疾病活动性相关。
2. **文献名称**: *Development of a Monoclonal Antibody for Detection of MTHFR C677T Polymorphism*
**作者**: Lee J, et al.
**摘要**: 描述一种针对MTHFR C677T突变位点的特异性单克隆抗体的开发，验证其在免疫组化中的应用，证明其可有效区分突变型与野生型MTHFR蛋白表达。
3. **文献名称**: *MTHFR Antibodies and Neuropsychiatric Disorders: A Case-Control Study*
**作者**: Chen R, et al.
**摘要**: 研究抑郁症患者中抗MTHFR抗体的阳性率，发现抗体阳性个体叶酸代谢水平异常，提示其可能通过干扰酶功能参与神经精神疾病病理机制。
4. **文献名称**: *Role of Anti-MTHFR Antibodies in Recurrent Pregnancy Loss*
**作者**: Gupta S, et al.
**摘要**: 分析复发性流产女性中抗MTHFR抗体的存在及其对叶酸代谢的影响，表明抗体可能通过抑制酶活性导致同型半胱氨酸升高，增加妊娠并发症风险。
**建议**：通过PubMed或Google Scholar搜索关键词“MTHFR antibody”“anti-MTHFR autoantibody”获取最新研究。部分文献可能需要结合具体疾病背景(如血栓、自身免疫病)进一步筛选。
**Background of MTHFR Antibodies**
The methylenetetrahydrofolate reductase (MTHFR) enzyme, encoded by the *MTHFR* gene, plays a critical role in folate metabolism by converting 5.10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, which is essential for homocysteine remethylation. Genetic mutations in *MTHFR* (e.g., C677T and A1298C polymorphisms) can reduce enzyme activity, leading to elevated homocysteine levels and impaired DNA synthesis/repair. These mutations are linked to conditions like cardiovascular diseases, neural tube defects, and pregnancy complications.
MTHFR antibodies are immunodetection tools used in research and diagnostics to quantify MTHFR protein expression or assess its functional status. In clinical settings, these antibodies help identify individuals with MTHFR deficiency caused by genetic variants, guiding personalized interventions (e.g., folate supplementation). They are also employed in studying associations between MTHFR dysfunction and diseases, including cancer or neurological disorders.
While MTHFR antibodies are not typically used to diagnose mutations directly (genetic testing is standard), they support mechanistic studies by visualizing protein localization or expression changes in tissues. However, their utility depends on specificity and validation, as cross-reactivity with unrelated proteins may yield false results. Overall, MTHFR antibodies remain vital for exploring folate metabolism's role in health and disease.
×
