| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 51 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human GSK3A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及GSK3A抗体的研究文献概览：
1. **"Characterization of a novel GSK3α-specific monoclonal antibody and its role in Alzheimer’s disease research"**
- **作者**: Hernandez et al. (2020)
- **摘要**: 开发了一种高特异性GSK3α单克隆抗体，验证了其在人脑组织Western blot和免疫组化中的适用性，发现阿尔茨海默病患者大脑中GSK3α的磷酸化水平与tau蛋白过度磷酸化显著相关。
2. **"GSK3 isoform-specific functions in glucose homeostasis: Insights from GSK3α antibody-based tissue profiling"**
- **作者**: Patel & Williams (2018)
- **摘要**: 通过GSK3α特异性抗体分析小鼠肝脏、肌肉组织，揭示GSK3α(而非GSK3β)在胰岛素信号通路中调控糖原合成的独特作用，为糖尿病治疗靶点提供依据。
3. **"Antibody-mediated detection of GSK3A interactions in Wnt signaling pathways"**
- **作者**: Liu et al. (2019)
- **摘要**: 利用共免疫沉淀(Co-IP)结合质谱技术，通过GSK3A抗体鉴定出结肠癌细胞中GSK3α与β-catenin的新型互作蛋白，证明其通过非经典Wnt通路影响肿瘤转移。
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*注：上述文献为示例性质，实际研究中建议通过PubMed或Web of Science以“GSK3A antibody”及相关关键词检索最新文献。*
Glycogen synthase kinase 3 alpha (GSK3A) is a serine/threonine protein kinase belonging to the GSK3 family, which includes the closely related isoform GSK3B. GSK3A plays crucial roles in cellular processes such as glycogen metabolism, cell proliferation, differentiation, and apoptosis. Unlike GSK3B, GSK3A has a unique N-terminal extension, but both isoforms share a conserved kinase domain and regulate downstream targets through phosphorylation, often in a primed (phosphorylation-dependent) manner. GSK3A activity is modulated by inhibitory phosphorylation at Ser21. mediated by upstream kinases like AKT, and is implicated in pathways including Wnt/β-catenin, insulin signaling, and neurodevelopment.
Antibodies targeting GSK3A are essential tools for studying its expression, localization, and post-translational modifications. They enable detection in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Many GSK3A antibodies are designed to distinguish between the phosphorylated (inactive) and unphosphorylated (active) forms, aiding in functional studies. Specificity is critical due to high homology between GSK3A and GSK3B, requiring validation using knockout controls or isoform-specific peptides. Applications span cancer research, neurodegenerative diseases (e.g., Alzheimer’s), and metabolic disorders (e.g., diabetes), where GSK3A dysregulation is linked to pathogenesis. Reliable antibodies support mechanistic insights and therapeutic target validation for small-molecule inhibitors.
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